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RNAseq analysis of B cells inducible for murine gammaherpesvirus 68 reactivation

ABSTRACT: RTA, the viral Replication and Transcription Activator, is essential for rhadinovirus lytic gene expression upon de novo infection and reactivation from latency. LPS/TLR4 engagement enhances rhadinovirus reactivation. We developed the HE-RIT cell line, a latent murine A20 B cell inducible for Flag-RTA expression and murine gammaherpesvirus 68 reactivation. LPS acted as a second stimulus to drive virus reactivation from latency in the context of induced expression of FLAG-RTA. We applied RNAseq to examine for genome-wide changes in viral gene expression in response to doxycycline-induced RTA-FLAG, alone or in combination with LPS.

ORGANISM(S): Mus musculus

PROVIDER: GSE94238 | GEO | 2017/02/18

SECONDARY ACCESSION(S): PRJNA369112

REPOSITORIES: GEO

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