Project description:Larvae expressing CD:UPRT under control of 109(2)80-Gal4 were fed 5EC at early L3 stage, prior to carcass dissection and RNA extraction. Reference RNA was prepared from carcasses of stage-matched larvae fed EUd and mock RNA was prepared from carcasses of
Project description:Whole blood was collected from healthy adult and peripheral blood mononuclear cells (PBMC) were isolated. PBMCs were cultured in medium or stimulated with RNase treated EC-12 or untreated EC-12 for 6hours. Then transcriptome profiles in PBMC were obtained.
Project description:For each experiment (as described in Pauli et al. 2006): Channel 1: whole worm lysate from strain SD1084 carrying transgene Pges-1::FLAG::PAB-1 Channel 2: mRNA immunoprecipitated from strain SD1084 carrying transgene Pges-1::FLAG::PAB-1 mRNA was amplified and hybridized to C. elegans DNA arrays from the Stuart Kim lab Set of arrays that are part of repeated experiments Keywords: Biological Replicate, mRNA tagging