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Transcriptional landscape of the human cell cycle

ABSTRACT: Steady-state gene expression across the cell cycle has been studied extensively. However, 2 transcriptional gene regulation and histone modification dynamics at different cell cycle stages is3 largely unknown. By applying a combination of GRO-seq, RNA-seq and histone modification 4 ChIP-seq, we depicted a comprehensive transcriptional landscape at G0/G1, G1/S and M phases 5 of breast cancer MCF-7 cells. Importantly, GRO-seq and RNA-seq analysis identified different 6 cell cycle regulated genes, suggesting a lag between transcription and steady-state expression 7 during the cell cycle. Interestingly, we identified genes actively transcribed at early M phase that 8 are longer in length and have low expression, which is accompanied by global increase of active 9 histone modifications of H3K4me2 and H3K27ac. In addition, we identified 2,440 cell cycle 10regulated enhancer RNAs (eRNAs) that are strongly associated with differential active 11transcription but not stable expression levels across the cell cycle. Motif analysis on dynamic 12eRNAs predicted KLF4 as a key regulator of G1/S transition, which was experimentally 13validated. Altogether, our combined analysis characterized the transcriptional and histone 14modification profile of the human cell cycle and identified novel dynamic transcriptional 15signatures across the cell cycle.

ORGANISM(S): Homo sapiens

PROVIDER: GSE94479 | GEO | 2017/03/16

SECONDARY ACCESSION(S): PRJNA369803

REPOSITORIES: GEO

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