A B-ARR-mediated cytokinin transcriptional network directs hormone-cross regulation and shoot development

ABSTRACT: While the biosynthesis, degradation, and signaling pathways for the essential plant hormone cytokinin have been extensively studied, the direct transcriptional targets of B-type ARRs, the key TFs essential for cytokinin action have not been defined. We epitope tagged four B-ARRs (1, 10, 12, and 14) using recombineering techniques and profiled TF binding sites using chromatin immunoprecipitation sequencing (ChIP-seq). The resulting cytokinin transcriptional response network allowed construction of the first cytokinin TF network, identification of numerous direct downstream targets along with the B-ARR-6BA motif and elucidation of one mechanism of activation of WUSCHEL, which requires a high concentration of cytokinin in shoot apical meristem for TF binding.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE94486 | GEO | 2018/04/25

REPOSITORIES: GEO