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Gene expression analysis in naïve and vaccine (peptide vs. DC) primed pmel-1 CD8 Tcells

ABSTRACT: We have shown that DC vaccine is superior to peptide vaccine in terms of priming and expansion of antigen-specific CD8+ T cells. DC vaccine-primed pmel-1 cells displayed better effecter functions than cells by peptide-primed cells in terms of cytokine production and externalization of cytotoxic granules. Furthermore DC vaccine-primed cells were metabolically distinct from peptide-primed cells. To confirm these findings, we performed a microarray analysis using splenic pmel-1 T cells from mice immunized with hgp100 peptide vaccine or DC vaccine. We also used splenic naïve pmel-1 T cells as a control.

ORGANISM(S): Mus musculus

PROVIDER: GSE94538 | GEO | 2017/08/31

SECONDARY ACCESSION(S): PRJNA371429

REPOSITORIES: GEO

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