Identification of the Sulfolobus acidocaldarius small RNome and L7Ae-RNA interactome

ABSTRACT: Small RNAs and L7Ae co-immunoprecipitated RNA were isolated from the thermophilic archaeon Sulfolobus acidocaldarius and subjected to Illumina sequencing. The sRNome revealed a high abundance of C/D box sRNAs and CRISPR RNAs. The L7Ae-RNA interactome showed enrichment of all known interactors of the L7Ae protein, i.e. C/D box sRNAs, H/ACA box sRNAs, RNaseP, rRNA. A high abundance of reads for the SRP RNA was found, suggesting L7Ae´s interaction with this universal RNA. Many mRNA reads were enriched, several of them comprise binding sites for the L7Ae protein.

ORGANISM(S): Sulfolobus acidocaldarius

PROVIDER: GSE94748 | GEO | 2017/02/10

SECONDARY ACCESSION(S): PRJNA373915

REPOSITORIES: GEO