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Quantification of differential gene expression by multiplexed targeted resequencing of cDNA

ABSTRACT: We present an approach for quantification of differential mRNA expression by targeted resequencing of cDNA using single-molecule molecular inversion probes (cDNA-smMIPs), which enable highly multiplexed resequencing of cDNA target regions of ~100 nt and counting of individual molecules. We show that accurate estimates of differential expression can be obtained from molecule counts for hundreds of smMIPs per reaction and that smMIPs are also suitable for quantification of relative gene expression and allele-specific expression. cDNA-smMIPs are a cost-effective tool for hypothesis-driven expression analysis in large numbers of genes (10 to 500) and samples (hundreds to thousands).

ORGANISM(S): synthetic construct Homo sapiens

PROVIDER: GSE94800 | GEO | 2017/03/01

SECONDARY ACCESSION(S): PRJNA374355

REPOSITORIES: GEO

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