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Generation of mature T cells from human hematopoietic stem/progenitor cells in artificial thymic organoids

ABSTRACT: Mechanistic studies of human T cell development require robust model systems that recapitulate the full span of thymopoiesis, from hematopoietic stem and progenitor cells (HSPC) through to mature naïve T cells. We developed a serum free, artificial thymic organoid (ATO) system that supports highly efficient and reproducible in vitro differentiation and positive selection to generate conventional human T cells from all sources of HSPCs. Deep-sequencing of the TCR variable genes was performed to compare the diversity of the TCR repertoire between ATO-derived CD8SP T cells and naive CD8SP T cells from thymus and blood.

ORGANISM(S): Homo sapiens

PROVIDER: GSE94968 | GEO | 2017/02/17

SECONDARY ACCESSION(S): PRJNA374973

REPOSITORIES: GEO

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