Transcriptomics

Dataset Information

0

HybridPoplar_H11-11_Leaves_MalacosomaDisstria_Herbivory


ABSTRACT: From a set of 15 standard, normalized or full-length cDNA libraries, we generated 139 007 3'- or 5'-end sequenced ESTs, representing more than one-third of the c. 385 000 publicly available Populus ESTs. Clustering and assembly of 107 519 3'-end ESTs resulted in 14 451 contigs and 20 560 singletons, altogether representing 35 011 putative unique transcripts, or potentially more than three-quarters of the predicted c. 45 000 genes in the poplar genome. Using this EST resource, we developed a cDNA microarray containing 15 496 unique genes, which was utilized to monitor gene expression in poplar leaves in response to herbivory by forest tent caterpillars (Malacosoma disstria). After 24 h of feeding, 1191 genes were classified as up-regulated, compared to only 537 down-regulated. Functional classification of this induced gene set revealed genes with roles in plant defence (e.g. endochitinases, Kunitz protease inhibitors), octadecanoid and ethylene signalling (e.g. lipoxygenase, allene oxide synthase, 1-aminocyclopropane-1-carboxylate oxidase), transport (e.g. ABC proteins, calreticulin), secondary metabolism [e.g. polyphenol oxidase, isoflavone reductase, (–)-germacrene D synthase] and transcriptional regulation [e.g. leucine-rich repeat transmembrane kinase, several transcription factor classes (zinc finger C3H type, AP2/EREBP, WRKY, bHLH)]. This study provides the first genome-scale approach to characterize insect-induced defences in a woody perennial providing a solid platform for functional investigation of plant–insect interactions in poplar. Keywords: stress response

ORGANISM(S): Populus Populus trichocarpa x Populus deltoides

PROVIDER: GSE9522 | GEO | 2008/04/22

SECONDARY ACCESSION(S): PRJNA103327

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2008-04-22 | E-GEOD-9522 | biostudies-arrayexpress
2008-12-31 | GSE14081 | GEO
2008-12-31 | GSE13767 | GEO
2008-12-30 | E-GEOD-13767 | biostudies-arrayexpress
2009-06-03 | GSE16365 | GEO
2009-06-03 | GSE16366 | GEO
| PRJNA824522 | ENA
2010-09-09 | E-GEOD-14081 | biostudies-arrayexpress
2006-09-22 | GSE3366 | GEO
2007-11-30 | GSE6403 | GEO