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Genomic characterization of murine monocytes reveals C/EBPb dependence of Ly6C-cells [RNA sequence]

ABSTRACT: We show that monocyte development is accompanied by stepwise RNA expression changes and that Ly6C+ monocytes upon transfer develop into Ly6C- monocytes indicated by a comparable expression profile to host Ly6C- monocytes

ORGANISM(S): Mus musculus

PROVIDER: GSE95411 | GEO | 2017/05/15

SECONDARY ACCESSION(S): PRJNA377059

REPOSITORIES: GEO

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Genomic characterization of murine monocytes reveals C/EBPb dependence of Ly6C-cells [ChIP data]

Project description:Chromatin status of murine blood Ly6C+ and Ly6C- monocytes indicates that both population share K4me3 marks (promoters) while Ly6C- monocytes establish de novo enhancers (K4me1, K4me2) during differentiation

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Genomic characterization of murine monocytes reveals C/EBPb dependence of Ly6C-cells [Single Cell RNA sequence]

Project description:Single cell RNA sequencing of murine circulating blood monocytes under steady state conditions. 2 plates of cx3cr1-cre:rosa26YFP monocytes and 4 plates (3 plates total monocytes and 1 plate Ly6Cint monocytes) were pre-enriched by CD115-biotin MACS and afterwards FACS sorted.

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Genomic characterization of murine monocytes reveals C/EBPb dependence of Ly6C-cells

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Genomic characterization of murine monocytes reveals C/EBPb dependence of Ly6C-cells [ATAC data]

Project description:Analysis of open chromatin by ATAC sequencing in murine blood monocyte subsets

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AffyExon1 genearray expression profile of adult mouse microglia and splenic Ly6C monocytes

Project description:Gene profiling of CNS-derived microglia vs splenic CD11b+Ly6C+ monocyte subsets deom adult mice Gene array identified 1572 genes that were enriched in microglia vs. 611 monocyte enriched genes with a greater than 5-fold difference (P<0.001). Gene profiling of CD11b+CD45Low microglia isolated from the CNS and CD11b+Ly6C+ monocyte subsets isolated from the spleen of naM-CM-/ve adult mice.

2013-07-08 | E-GEOD-48579 | biostudies-arrayexpress

Genomic characterization of murine monocytes reveals C/EBPb dependence of Ly6C-cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2017-05-15 | GSE95702 | GEO

Heterogeneity of Ly6G+Ly6C+ myeloid-derived suppressor cell (MDSC) infiltrates during S. aureus biofilm infection

Project description:The two immune cell populations Myeloid-derived suppressor cells (MDSCs), monocytes (MONO) and neutrophils (PMNs) are difficult to differentiate because of shared surface marker expression. Here we utilize the integrin receptor CD11b combined with conventional Ly6G and Ly6C expression to more accurately separate cellular populations via FACS. Then we apply high-throughput RNA Sequencing to Ly6G+Ly6C+CD11bhigh MDSC, Ly6G+Ly6C+CD11blow PMN and Ly6G-Ly6C+ monocyte populations. A total of 6,466 genes were significantly differentially expressed in MDSCs vs. monocytes, whereas only 297 genes were significantly different between MDSCs and PMNs. A number of genes implicated in cell cycle regulation were identified, and in vivo EdU labeling revealed that over 75% of MDSCs proliferated locally at the site of S. aureus biofilm infection.

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Expression data from mouse circulating Ly6C-low subset of monocytes (patrolling monocytes)

Project description:Expression data from mouse circulating Ly6C-low subset of monocytes (patrolling monocytes)

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Expression arrays of inflammatory Ly6C+ monocytes in mice primary or secondary challenged with Listeria monocytogenes

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G-CSF mobilizes CD34+ regulatory monocytes that inhibit Graft-versus-Host Disease

Project description:Administration of G-CSF mobilizes a unique population of CD11b+Ly6C+CD34+mature monocytes that can inhibit GVHD in murine models of BMT via an iNOS-dependent mechanism. The transcriptional profiles of flow sorted lineage-CD11b+CD34+ cells from G-CSF treated mice were compared with conventional splenic Ly6C+ and Ly6C- monocytes, progenitor cells and cultured myeloid-derived suppressor cells. Further comparisons were made with lineage-CD11b+CD34+ cells from G-CSF treated mice that had been grown in culture or that were derived from iNOS ko mice. We used microarrays to detail the global programme of gene expression underlying diffrenetiation of each of these cell types Lin-CD11b+CD34+ populations were isolated directly from the spleens of G-CSF-treated C57BL/6 mice or iNOS ko mice. In untreated C57BL/6 mice, Lin-CD11b+CD115+Ly6C+ and Lin-CD11b+CD115+Ly6C- monocytes were isolated from the spleen and Lin-CD117+CD115+CD135-Ly6C+CD11b- common monocyte progenitors were isolated from the bone marrow. Myeloid-derived suppressor cells (Ly6C+CD11b+ cells derived from G-CSF, GM-CSF and IL-13 cultured C57BL/6 bone marrow) were also isolated and compared with the above populations. Lin-CD11b+CD34+ spleen cells derived from G-CSF-treated C57BL/6 mice were cultured for 3 days in Flt3 ligand and SCF and then compared to the original input population.

2015-04-01 | E-GEOD-65670 | biostudies-arrayexpress

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