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Genomic characterization of murine monocytes reveals C/EBPb dependence of Ly6C-cells [RNA sequence]

ABSTRACT: We show that monocyte development is accompanied by stepwise RNA expression changes and that Ly6C+ monocytes upon transfer develop into Ly6C- monocytes indicated by a comparable expression profile to host Ly6C- monocytes

ORGANISM(S): Mus musculus

PROVIDER: GSE95411 | GEO | 2017/05/15

SECONDARY ACCESSION(S): PRJNA377059

REPOSITORIES: GEO

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Genomic characterization of murine monocytes reveals C/EBPb dependence of Ly6C-cells [ChIP data]

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Genomic characterization of murine monocytes reveals C/EBPb dependence of Ly6C-cells [Single Cell RNA sequence]

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Genomic characterization of murine monocytes reveals C/EBPb dependence of Ly6C-cells

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Project description:Analysis of open chromatin by ATAC sequencing in murine blood monocyte subsets

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2013-07-08 | E-GEOD-48579 | biostudies-arrayexpress

Genomic characterization of murine monocytes reveals C/EBPb dependence of Ly6C-cells

Project description:This SuperSeries is composed of the SubSeries listed below.

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Expression data from mouse circulating Ly6C-low subset of monocytes (patrolling monocytes)

Project description:Expression data from mouse circulating Ly6C-low subset of monocytes (patrolling monocytes)

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Heterogeneity of Ly6G+Ly6C+ myeloid-derived suppressor cell (MDSC) infiltrates during S. aureus biofilm infection

Project description:The two immune cell populations Myeloid-derived suppressor cells (MDSCs), monocytes (MONO) and neutrophils (PMNs) are difficult to differentiate because of shared surface marker expression. Here we utilize the integrin receptor CD11b combined with conventional Ly6G and Ly6C expression to more accurately separate cellular populations via FACS. Then we apply high-throughput RNA Sequencing to Ly6G+Ly6C+CD11bhigh MDSC, Ly6G+Ly6C+CD11blow PMN and Ly6G-Ly6C+ monocyte populations. A total of 6,466 genes were significantly differentially expressed in MDSCs vs. monocytes, whereas only 297 genes were significantly different between MDSCs and PMNs. A number of genes implicated in cell cycle regulation were identified, and in vivo EdU labeling revealed that over 75% of MDSCs proliferated locally at the site of S. aureus biofilm infection.

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Expression arrays of inflammatory Ly6C+ monocytes in mice primary or secondary challenged with Listeria monocytogenes

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G-CSF mobilizes CD34+ regulatory monocytes that inhibit Graft-versus-Host Disease

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