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Prepartal energy intake alters blood polymorphonuclear leukocyte transcriptome during the peripartal period in Holstein cows

ABSTRACT: Using an Agilent microarray platform, the present study examined changes in the transcriptome of bovine polymorphonuclear leukocytes (PMNL) due to prepartal dietary intake. Twelve Holstein cows were fed a high-straw, control diet (CON; NEL = 1.34 Mcal/kg) or overfed a moderate-energy diet (OVE; NEL = 1.62 Mcal/kg) during the dry period. Blood for PMNL isolation and metabolite analysis was collected at -14 and +7 d relative to parturition. At an ANOVA false discovery rate (FDR) <0.05, energy intake (OVE vs. CON) influenced 1806 genes. Dynamic Impact Approach (DIA) bioinformatics analysis classified treatment effects on KEGG pathways, including: activated Oxidative Phosphorylation and Biosynthesis of Unsaturated Fatty Acids, and inhibited RNA Polymerase, Proteasome, and Toll-like Receptor Signaling Pathway. This analysis indicates that processes critical for energy metabolism and cellular and immune function were impacted with mixed results. However, overall interpretation of the transcriptome data agreed in part with literature documenting a potentially detrimental, chronic activation of PMNL in response to overfeeding. The widespread, transcriptome-level changes captured here confirm the importance of dietary energy adjustments around calving on the immune system.

ORGANISM(S): Bos taurus

PROVIDER: GSE95677 | GEO | 2017/03/31

SECONDARY ACCESSION(S): PRJNA377893

REPOSITORIES: GEO

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Prepartal energy intake alters blood polymorphonuclear leukocyte transcriptome during the peripartal period in Holstein cows

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