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Chromatin accessibility profiling identifies cis-regulatory modules in an early embryonic cell lineage

ABSTRACT: We used DNase-seq and ATAC-seq to identify a set of putative CRMs regulating skeletogenic gene expression during sea urchin embryogenesis. These CRMs, identified by differential chromatin accessibility, are likely to be regulated by two upstream TFs, Alx1 and Ets1, that are part of the skeletogenic GRN. 9/31 od these identified CRMs were validated by reporter gene assay. Our work demonstrates the value of using differential chromatin accessibility for the high-throughput identification of CRMs in embryonic tissues.

ORGANISM(S): Strongylocentrotus purpuratus

PROVIDER: GSE96927 | GEO | 2018/04/01

REPOSITORIES: GEO

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