Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data from DMOS- or paeoniflorin- treated human pancreatic cancer cell line Capan-1

ABSTRACT: Paeoniflorin (PF) is an active monoterpene glycoside extracted from Paeonia lactiflora Pall. Recent studies showed that PF has anti-tumor effects in various types of cancer. However, the function of PF in pancreatic cancer (PA) is largely unexplored. Here, we showed that PF suppressed growth of PA cell lines Capan-1 and MIAPaCa-2, and profoundly sensitized these cells to X-ray irradiation. Through a microarray analysis, we identified tumor-suppressor candidate gene HTRA3 as the most increased gene upon PF treatment in Capan-1 cells. Ectopic expression of HTRA3 led to reduced cell growth and increased expression of apoptotic protein Bax, suggesting a tumor suppressive role of HTRA3 in PA cells. Together, our results provide a set of genetic and biologic proofs that PF inhibited PA cell growth by upregulation of HTRA3.

ORGANISM(S): Homo sapiens

PROVIDER: GSE97124 | GEO | 2017/09/20

SECONDARY ACCESSION(S): PRJNA380866

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Paeonia lactiflora extract suppresses cisplatin-induced muscle wasting via donwnregulations of muscle-specific E3 ubiquitin ligases

Project description:Cisplatin is a broad-spectrum anti-cancer drugs used in diverse types of cancer, however, muscle wasting is one of the most common side effects of cisplatin chemotherapy. This study aimed to elucidate the effects of an ethanol extract of the root of Paeonia lactiflora Pall. (Radix Paeoniae, RP) on cisplatin-induced muscle wasting along with its molecular mechanism.

2020-03-28 | GSE147613 | GEO

Identification of genes responsive to paeoniflorin, a heat shock protein-inducing compound, in human leukemia U937 cells

Project description:Paeoniflorin (PF) isolated from paeony root (Paeoniae radix) has been used as an herbal medicine in East Asis for its anti-allergic, anti-inflammatory, and immunoregulatory effects. PF is known to be a chemical heat shock protein (HSP) inducer. The effects on the gene expression in human lymphoma U937 cells treated with PF were investigated using by an Affymetrix GeneChip system. PF treatment induced Hsp70 expression in U937 cells in a dose- and time-dependent manner as shown in Western blot analysis. When the cells were treated with PF (160 μg/ml; 30 min), 41 up-regulated and 23 down-regulated genes were identified. Keywords: paeoniflorin, gene expression, Human lymphoma U937 cell

2007-06-23 | GSE8228 | GEO

Integration of transcriptome, small RNAs and degradome sequencing uncovers a tissue-specific regulatory network of paeoniflorin biosynthesis in Paeonia Lactiflora Pall. [miRNA-seq]

Project description:The study of paeoniaflorin biosynthesis in Paeonia lactiflora Pall.at a molecular level is scarce, and the regulatory network between small RNAs and mRNAs in paeoniaflorin biosynthesis has not been assessed. Here, we attempt to combine mRNA and miRNA expression data with degradome analysis to reveal the tissue-specific regulatory network of miRNAs and their targets in Paeonia, especially the complex network of paeoniaflorin biosynthesis.The results will advance our understanding of the miRNA-mediated molecular mechanisms of paeoniaflorin and monoterpenoids biosynthesis, and provide a valuable resource for further study on Paeonia.

2021-03-03 | GSE167994 | GEO

Integration of transcriptome, small RNAs and degradome sequencing uncovers a tissue-specific regulatory network of paeoniflorin biosynthesis in Paeonia Lactiflora Pall. [RNA-seq]

2021-03-03 | GSE167993 | GEO

Project description:Root of Paeonia lactiflora Pall.

| PRJNA665305 | ENA

Paeonia lactiflora suppressed cancer-cachexia by downregulating muscular NF-κB signaling and muscle-specific E3 ubiquitin ligases in cancer-bearing mice.

Project description:Paeonia lactiflora restored skeletal muscle function and mass in cancer-bearing mice, through the downregulations of muscular NF-kB signalling and muscle-specific E3 ubiquitin ligases. This study provides experimental evidence that Paeonia lactiflora can be a plausible candidate for a therapeutic agent for cancer cachexia.

2019-06-04 | GSE132120 | GEO

Project description:Transcriptome of Paeonia lactiflora Pall. tissues

| PRJNA656429 | ENA

Integration of transcriptome, small RNAs and degradome sequencing uncovers a tissue-specific regulatory network of paeoniflorin biosynthesis in Paeonia Lactiflora Pall.

Project description:Integration of transcriptome, small RNAs and degradome sequencing uncovers a tissue-specific regulatory network of paeoniflorin biosynthesis in Paeonia Lactiflora Pall.

| PRJNA705715 | ENA

Integration of transcriptome, small RNAs and degradome sequencing uncovers a tissue-specific regulatory network of paeoniflorin biosynthesis in Paeonia Lactiflora Pall. [RNA-seq]

Project description:Integration of transcriptome, small RNAs and degradome sequencing uncovers a tissue-specific regulatory network of paeoniflorin biosynthesis in Paeonia Lactiflora Pall. [RNA-seq]

| PRJNA705718 | ENA

Integration of transcriptome, small RNAs and degradome sequencing uncovers a tissue-specific regulatory network of paeoniflorin biosynthesis in Paeonia Lactiflora Pall. [miRNA-seq]

| PRJNA705720 | ENA