Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

P21 misexpression in the left growth plates

ABSTRACT: To study mechanisms of long bone growth regulation, p21 misexpression was induced in the left hindlimb of mouse embryos using an intersectional approach that requires both Cre and (r)tTA activity. Doxycycline was provided to the pregnant female at embryonic day (E)12.5 to activate the transgene, and embryos were collected at E17.5. Distal femur and proximal tibia growth plates were dissected out, keeping left and right separated, deprived of perichondrium and flash frozen. After RNA extraction, mRNA libraries were prepared and all samples were deep sequenced in parallel

ORGANISM(S): Mus musculus

PROVIDER: GSE97232 | GEO | 2018/06/27

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Expression data from growth plate cartilage of control and immobilized embryonic chickens

Project description:Affymetrix Chicken Gene 1.0 ST Array (ChiGene-1_1-st) profiles were generated from growth plate cartilage derived from the distal femur and proximal tibia of embryonic chickens at three developmental stages (day 12, 13 and 15) corresponding to stages prior to and immediately after the acqusition of mechano-responsive cartilage growth in the femur but not tibia (which does not respond to mechanical stimuli until later stages). Additionally, array profiles were generated from growth plate cartilage from the distal femur and proximal tibia of E15 chicks which were pharmacologically immobilized by decamethonium bromide treatment, which reduces longitudinal growth of the femur only at this stage.

2016-12-31 | GSE77412 | GEO

Specialized post-arterial capillaries for adult bone remodeling [BoneEC]

Project description:A single cell transcriptomic profile of bone endothelial cells of the murine femur and tibia.

2024-08-22 | GSE239624 | GEO

Transcriptional profiling of long bone skeletal stem cells and vertebral counterparts

Project description:To identify specific markers for vertebral skeletal stem cells (vSSCs), we isolated vSSC candidates and lbSSCs by FACS from p10 mice long bone (tibia and femur) and vertebrae.

2023-05-05 | GSE230852 | GEO

Gene expression profile of bone marrow derived M-CSF-dependent macrophage from Ogfrl1 KO mouse

Project description:To investigate the role of Ogfrl1 in macrophages, we isolated bone marrow derived M-CSF-dependent macrophages (BMMs) from femur and tibia of Ogfrl1 deficient mouse and wildtype littermate mouse.

2023-12-01 | GSE247888 | GEO

Chicken ceca, blood, tibia, and femur

Project description:Chicken ceca, blood, tibia, and femur Raw sequence reads

| PRJNA486024 | ENA

ER, mitochondria and ISR regulation by mt-HSP70 and ATF5 upon procollagen misfolding (E18.5 femur and tibia)

Project description:We used single cell RNA sequencing (scRNA-seq) to identify femur and tibia osteoblast cell stress response pathway to misfolding of type I procollagen with Gly610->Cys substitution in the triple helical region of alpha2(I) chain.

2022-08-07 | GSE210511 | GEO

Gene expression profile at single cell level of Gli1+ progenitors from the metaphysis of distal femur and proximal tibia in response to MPS.

Project description:Gli1+ progenitors are considered as metaphyseal mesenchymal progenitors in the distal femur and proximal tibia under the growth plate. We used single cell RNA sequencing (scRNA-seq) to analyze the diversity of Gli1+ progenitors in response to methylprednisolone.

2024-04-16 | GSE248553 | GEO

Genome-wide analysis of cartilage gene expression in response to cartilage-specific ablation of Site-1 protease (Mbtps1) in mice (S1Pcko mice)

Project description:Analysis of differential gene expression in chondrocytes due to S1P ablation in mice. The hypothesis tested in this study was that ablation of S1P in mice would result in differential gene expression in chondrocytes that may be responsible for the S1Pcko phenotype. The chondroepiphyseal cartilage from the tip of appendicular bones such as femur, tibia, humerus, radius, ulna were harvested from E16.5 wild type (WT) and S1Pcko embryos. In addition, the appendicular bones and calvarium from E16.5 WT and S1Pcko embryos also were harvested and processed for total RNA without removing the chondroepiphyseal cartilage. The chondroepiphyseal cartilage and bones were rolled on Whatman Filter paper to remove muscle and blood vessels, and total RNA was extracted, labeled, hybridized on Illumina Mouse WG-6 v1.1 and analyzed to identify genes that were differentially expressed in the S1Pcko skeletal elements when compared to WT.

2014-07-31 | E-GEOD-55577 | biostudies-arrayexpress

Transcription profiling of 5 heart chambers of mice 20 days after Thoracic Aortic Constriction (TAC) which led to left atrial enlargement and left ventricular hypertrophy

Project description:15 ug RNA isolated from 5 heart chambers of mice 20 days after Thoracic Aortic Constriction which led to left atrial enlargement and left ventricular hypertrophy was used to prepare labeled cDNA for test channel, 15ug of RNA from whole e17.5 mouse embryos used to prepare labeled cDNA for reference channel. 3 biological replicates were performed for eash tissue. Diseased chamber tissue was compared to sham operated control tissue.

2005-01-01 | E-SMDB-24 | biostudies-arrayexpress

Profiling the transcriptome of the adult mouse skeleton: RNA-seq of osteocytes from tibiae, femora, humeri and calvariae

Project description:In this experiment we define the transcriptome of the adult mouse skeleton by performing total-RNA transcriptome-sequencing on osteocytes, the critical regulatory cells in bone. Osteocytes from 16 week-old male mice (n=8) were isolated for 4 bone types across the skeleton: the tibia, femur, humerus, and calvaria. All other soft tissues including marrow were removed from samples before sequencing.

2020-12-16 | E-MTAB-5532 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data