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Characterization of mammalian NEF-sp as a testis-specific nuclear 3′→5′ exoribonuclease

ABSTRACT: Ribonucleases catalyse maturation of functional RNAs or mediate degradation of cellular transcripts, activities that are critical for gene expression control. Here we identify a previously uncharacterized mammalian nuclease family member NEF-sp (LOC81691) as a testis-specific factor. Recombinant human NEF-sp demonstrates a divalent metal ion-dependent 3′→5′ exoribonuclease activity. This activity is specific to single-stranded RNA substrates and is independent of their length. Presence of a 2′-O-methyl modification at the 3′ end of the RNA substrate is inhibitory. Ectopically-expressed NEF-sp localizes to the nucleolar/nuclear compartment in human cell cultures and this is dependent on an N-terminal nuclear localization signal. Finally, mice lacking NEF-sp are viable and display normal fertility, likely indicating overlapping functions with other nucleases. Taken together, our study provides the first biochemical and genetic exploration of the role of the NEF-sp exoribonuclease in the mammalian genome.

ORGANISM(S): Mus musculus

PROVIDER: GSE97235 | GEO | 2017/07/04

SECONDARY ACCESSION(S): PRJNA381044

REPOSITORIES: GEO

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Mus musculus

Project description:Characterization of mammalian NEF-sp as a testis-specific nuclear 3′→5′ exoribonuclease

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