Transcriptomics

Dataset Information

0

Gene Expression in S49 Deathless (D-) cell variant


ABSTRACT: The second messenger cAMP acts via protein kinase A (PKA) to induce apoptosis by mechanisms that are poorly understood. Here, we assessed a role for mitochondria and analyzed gene expression in cAMP/PKA-promoted apoptosis by comparing wild-type (WT) S49 lymphoma cells and the S49 variant, D- (cAMP-deathless), which lacks cAMP-promoted apoptosis but has wild-type levels of PKA activity and cAMP-promoted G1 growth arrest. Treatment of WT, but not D-, S49 cells with 8-CPT-cAMP for 24 h induced loss of mitochondrial membrane potential, mitochondrial release of cytochrome c and Smac and increase in caspase-3 activity. Gene expression analysis (using Affymetrix 430 2.0 Arrays) revealed that WT and D- cells incubated with 8-CPT-cAMP have similar, but non-identical, extents of cAMP-regulated gene expression at 2h (~800 transcripts) and 6h (~1000 transcripts) (|Fold|>2, P<0.06); by contrast, at 24h ~2500 and ~1100 transcripts were changed in WT and D- cells, respectively. Using an approach that combined regression analysis, clustering and functional annotation to identify transcripts that showed differential expression between WT and D- cells, we found differences in cAMP-mediated regulation of mRNAs involved in transcriptional repression, apoptosis, the cell cycle, RNA splicing, Golgi and lysosomes. The 2 cell lines differed in CREB phosphorylation and expression of the transcriptional inhibitor Icer and in cAMP-regulated expression of genes in the Inhibitor of apoptosis (IAP) and Bcl families. The findings indicate that cAMP/PKA-promoted apoptosis of lymphoid cells occurs via mitochondrial-mediated events and imply that such apoptosis involves gene networks in multiple biochemical pathways. Keywords: time course

ORGANISM(S): Mus musculus

PROVIDER: GSE9727 | GEO | 2007/12/12

SECONDARY ACCESSION(S): PRJNA103643

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2008-06-16 | E-GEOD-9727 | biostudies-arrayexpress
2007-08-31 | E-GEOD-2413 | biostudies-arrayexpress
2005-04-15 | GSE2413 | GEO
2010-01-01 | E-MTAB-122 | biostudies-arrayexpress
2010-11-24 | E-GEOD-25541 | biostudies-arrayexpress
2013-05-15 | E-MTAB-1662 | biostudies-arrayexpress
2021-08-24 | GSE182498 | GEO
2015-08-07 | E-GEOD-71252 | biostudies-arrayexpress
2022-10-14 | PXD032160 | Pride
2024-08-20 | GSE182413 | GEO