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Interplay between mitochondria and diet mediates pathogen and stress resistance in C. elegans.

ABSTRACT: To understand the impact of a diet on host health, we performed a number of assays with C. elegans reared on OP50 or HT115 E. coli. We observed that HT115 provided a substantial increase in resistance to Liquid Killing and abiotic stresses (e.g., heat shock, oxidative stress). This phenomenon was independent of the worms’ innate immune system. In the absence of any other obvious candidates, we turned to microarray transcriptional profiling to gain an unbiased picture of the differences that result from feeding C. elegans with HT115 instead of OP50. To our surprise, there was a relatively small number of genes differentially expressed between worms fed OP50 and HT115. Amongst them were several mitochondrially-associated genes, suggesting that the relevant difference may involve this organelle. Using a variety of approaches, we determined that worms fed HT115 exhibit increased mitochondrial health than their OP50 counterparts. Furthermore, supplementation of growing OP50 with cobalamin recapitulated virtually all of the HT115 phenotypes.

ORGANISM(S): Caenorhabditis elegans

PROVIDER: GSE97678 | GEO | 2019/02/21

REPOSITORIES: GEO

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