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DNA epigenome editing using CRISPR-Cas SunTag-directed DNMT3A [RRBS]

ABSTRACT: We demonstrate that dCas9-SunTag-DNMT3A dramatically increased CpG methylation at the HOXA5 locus in human embryonic kidney 293T cells (HEK293T). Furthermore, using a single sgRNA, dCas9-SunTag-DNMT3A was able to methylate a 4.5 kb genomic region and repress HOXA5 gene expression. Reduced representation bisulfite sequencing (RRBS) and RNA-seq showed that dCas9-SunTag-DNMT3A methylated regions of interest with minimal impact on the global DNA methylome and transcriptome.

ORGANISM(S): Homo sapiens

PROVIDER: GSE97813 | GEO | 2017/08/22

SECONDARY ACCESSION(S): PRJNA382988

REPOSITORIES: GEO

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DNA epigenome editing using CRISPR-Cas SunTag-directed DNMT3A [WGBS]

Project description:We demonstrate that dCas9-SunTag-DNMT3A dramatically increased CpG methylation at the HOXA5 locus in human embryonic kidney 293T cells (HEK293T). Furthermore, using a single sgRNA, dCas9-SunTag-DNMT3A was able to methylate a 4.5 kb genomic region and repress HOXA5 gene expression. Reduced representation bisulfite sequencing (RRBS) and RNA-seq showed that dCas9-SunTag-DNMT3A methylated regions of interest with minimal impact on the global DNA methylome and transcriptome.

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DNA epigenome editing using CRISPR-Cas SunTag-directed DNMT3A

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DNA epigenome editing using CRISPR-Cas SunTag-directed DNMT3A.

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