Transcriptomics

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Transcriptome-wide analysis of IL2RA enhancer activation by CRISPRa


ABSTRACT: We previously performed a tiling CRISPR activation (CRISPRa) screen in Jurkat T cells to discover enhancers controlling IL2RA expression. This screen identified six regions where recruitment of dCas9-VP64 was sufficient to drive increased expression of IL2RA. We named these putative enhancers CRISPRa Responsive Elements (CaREs). To examine transcriptome-wide consequences of dCas9-VP64 recruitment to IL2RA CaREs, we performed RNA-Seq on HuT78 cells stably expressing dCas9-VP64 and gRNAs targeting the IL2RA TSS, CaRE3, or CaRE4, or stimulated with anti-CD3/CD28 antibodies.

ORGANISM(S): Homo sapiens

PROVIDER: GSE98177 | GEO | 2017/04/26

SECONDARY ACCESSION(S): PRJNA384202

REPOSITORIES: GEO

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