Dataset Information

Expression data from breast cancer cells MDA-MB-231

ABSTRACT: CCCTC-binding factor (CTCF) is an important epigenetic regulator that has been implicated in multiple cellular processes including growth, proliferation, differentiation, and apoptosis. Deletion or mutation of CTCF is associated with human breast carcinogenesis. However, the exact role that CTCF plays in breast cancer has not been fully elucidated. To investigate the biological functions of CTCF in breast cancer and the underlying mechanisms, we carried out microarray analysis comparing the gene expression of CTCF overexpressing and control MDA-MB-231 cells to screen the possible target genes and pathways that CTCF regulated.

ORGANISM(S): Homo sapiens

PROVIDER: GSE98337 | GEO | 2017/05/01

SECONDARY ACCESSION(S): PRJNA384779

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:Since bone metastatic breast cancer is an incurable disease, causing significant morbidity and mortality, understanding of the underlying molecular mechanisms would be highly valuable. Here, we describe in vitro and in vivo evidence for the importance of serine biosynthesis in the metastasis of breast cancer to bone. We first characterized the bone metastatic propensity of the MDA-MB-231(SA) cell line variant as compared to the parental MDA-MB-231 cells by radiographic and histological observations in the inoculated mice. Genome-wide gene expression profiling of this isogenic cell line pair revealed that all the three genes involved in the L-serine biosynthesis pathway, phosphoglycerate dehydrogenase (PHGDH), phosphoserine aminotransferase 1 (PSAT1), and phosphoserine phosphatase (PSPH) were upregulated in the highly metastatic variant. This pathway is the primary endogenous source for L-serine in mammalian tissues. Consistently, we observed that the proliferation of MDA-MB-231(SA) cells in serine-free conditions was dependent on PSAT1 expression. In addition, we observed that L-serine is essential for the formation of bone resorbing human osteoclasts and may thus contribute to the vicious cycle of osteolytic bone metastasis. High expression of PHGDH and PSAT1 in primary breast cancer was significantly associated with decreased relapse-free and overall survival of patients and malignant phenotypic features of breast cancer. In conclusion, high expression of serine biosynthesis genes in metastatic breast cancer cells and the stimulating effect of L-serine on osteoclastogenesis and cancer cell proliferation indicate a functionally critical role for serine biosynthesis in bone metastatic breast cancer and thereby an opportunity for targeted therapeutic interventions. Parental MDA-MB-231 cells and MDA-MB-231(SA) cells were cultured in cell culture flasks. RNA was isolated in order to compare the gene expression profiles of these cell variants. Total of two samples. No replicates.

Dataset Information

Expression data from breast cancer cells MDA-MB-231

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets