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Novel small RNA spike-in oligonucleotides enable absolute normalization of small RNA-Seq data

ABSTRACT: Normalization of high-throughput small RNA sequencing (sRNA-Seq) data is required to compare sRNA levels across different samples. Commonly used relative normalization approaches can cause erroneous conclusions due to fluctuating small RNA populations between tissues. We developed a set of sRNA spike-in oligonucleotides (sRNA spike-ins) that enable absolute normalization of sRNA-Seq data across independent experiments, as well as the genome-wide estimation of sRNA:mRNA stoichiometries when used together with mRNA spike-in oligonucleotides.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE98553 | GEO | 2017/07/19

SECONDARY ACCESSION(S): PRJNA385496

REPOSITORIES: GEO

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