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Benign Uncultured CD133+ vs CD133-

ABSTRACT: 6 benign TURP samples taken directly from patients were subjected to collagen digestion overnight. Epithelial cells isolated were adhered to collagen for 15 minutes. Adherent cells were passed through a magnetic bead column and cells positive for CD133 antibody were double selected. RNA was extracted from CD133+ and CD133- cells from each patient and amplified using the RiboAmp HS RNA amplification system. CD133+ (Cy5) and CD133- (Cy5) cDNA was hybridised to 30K cDNA array (CRUK facility) using the Invitrogen post labelling system. In each case that RNA was co-hybridised to a human pooled universal reference cDNA (Cy3). Keywords: repeat sample

ORGANISM(S): Homo sapiens

PROVIDER: GSE9935 | GEO | 2008/03/01

SECONDARY ACCESSION(S): PRJNA105065

REPOSITORIES: GEO

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