Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Estradiol and ICI 182,780 infusions induce similar transcriptomic response in late gestation fetal hypothalamus

ABSTRACT: Estradiol plays a critical role stimulating the fetal hypothalamus-pituitary-adrenal axis at the end of gestation. Estradiol action is mediated through nuclear and membrane receptors that can be modulated by ICI 182,780, a pure anti-estrogen compound. The objective of this study was to evaluate the transcriptomics of estradiol and ICI 182,780, testing the hypothesis that ICI 182,780 blocks the action of estradiol in the fetal hypothalamus. However, we found that a short term (48 hrs) infusion with ICI 182,780 induces a similar transcriptomic response than estradiol infusion in the late gestation ovine fetal hypothalamus, being more evident with higher doses of ICI 182,780. These results suggest that ICI 182,780 is primarily an agonist of estradiol in the developing brain.

ORGANISM(S): Ovis aries

PROVIDER: GSE99497 | GEO | 2018/01/31

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Ovis aries

Project description:Estradiol and ICI 182,780 infusions induce similar transcriptomic response in late gestation fetal hypothalamus

| PRJNA388662 | ENA

Genomics of Estradiol-3-Sulfate Action in the Ovine Fetal Hypothalamus

Project description:In the fetal sheep during late gestation sulfoconjugated estrogens in plasma reach a concentration 40-100 times greater than unconjugated estrogens. The objective of the present study was to determine the genomics of estradiol-3-sulfate (E2S) action in the fetal brain. The hypothesis was that E2S stimulates genes involved in the neuroendocrine pathways in the hypothalamus that direct or facilitate fetal development at the end of gestation. Four sets of chronically-catheterized ovine twin fetuses were studied (gestational age: 120-127 days gestation) with one infused with E2S intracerebroventricularly (1 mg/day) and the other remained untreated (control). After euthanasia, mRNA samples were extracted from the 8 hypothalami, corresponding to the four treatment and four control fetuses. Microarray analysis was performed following the Agilent protocol for 1-color 8x15 microarrays, designed for Ovis aries. A total of 4 sets of chronically-catheterized ovine twin fetuses were studied with one infused with estradiol-3-sulfate intracerebroventricularly (1 mg/day) for 7-12 days, using an osmotic mini-pump implanted in the fetus, and the other served as an untreated control. The gestational age at the time of surgery was 120-127 days of gestation. Twin fetuses were randomly assigned to the two groups at the time of surgery. After 7-12 days of infusion, twin fetuses of known gestational age (130 to 134 days) were euthanized and mRNA samples were extracted from the 8 hypothalami, corresponding to the four treatment and four control fetuses.

2011-08-02 | E-GEOD-31184 | biostudies-arrayexpress

Effects of Triclosan exposition on fetal hypothalamic transcriptomics

Project description:Triclosan (TCS), an antibacterial compound commonly added to personal care products, could be an endocrine disruptor at low doses. Although TCS has been shown to alter fetal physiology, its effects in the developing fetal brain are unknown. The objective of this study was to use transcriptomics and systems analysis to determine significantly altered biological processes in the late gestation ovine fetal hypothalamus after direct or indirect exposure to low doses of TCS. We found that short-term infusion of TCS induces vigorous changes in the fetal hypothalamic transcriptomics, which are mainly related to food intake pathways and metabolism. For direct TCS exposure, chronically catheterized late gestation fetal sheep were infused with vehicle (n=4) or TCS (250 Î¼g/day; n=4) iv. For indirect TCS exposure, TCS (100 Î¼g/kg/day; n=3) or vehicle (n=3) was infused into the maternal circulation. Fetal hypothalami were collected after 2 days of infusion, and gene expression was measured using Agilent 15k ovine microarrays.

2016-02-29 | E-GEOD-69275 | biostudies-arrayexpress

Genomics of Estradiol-3-Sulfate Action in the Ovine Fetal Hypothalamus

2011-08-03 | GSE31184 | GEO

Transcription profiling of human primary osteoblasts treated with estradiol or the pure antagonist ICI 182,780a potent inhibitor of estrogen receptor signaling

Project description:Transcriptional profiling of estrogen regulated genes in human primary osteoblasts. The cells were either treated with estradiol (1 nM) or the pure antagonist ICI 182,780 (Faslodex), which acts as a potent inhibitor of estrogen receptor signaling.

2007-08-01 | E-MEXP-1053 | biostudies-arrayexpress

Ovis aries

Project description:Genomics of Estradiol-3-Sulfate Action in the Ovine Fetal Hypothalamus

| PRJNA144781 | ENA

Effects of Triclosan exposition on fetal hypothalamic transcriptomics

2016-02-29 | GSE69275 | GEO

Transcription profiling of human MCF-7 cells treated with Fulvestrant (ICI 182,780) and vehicle reveals origins and phenotypic variations of antiestrogen resistance in breast cancer

Project description:Expression profiles of MCF-7 cells treated with Fulvestrant (ICI 182,780) and vehicle Experiment Overall Design: MCF7 cells were grown in DMEM supplemented with 5% FCS (Hyclone, "Defined" grade). Growth medium was changed immediately before addition of 100 nM of ICI 182,780 or vehicle alone (0.1% ethanol) and cells incubated for 48h before they were harvested for profiling. Note that MCF7 cells show no signs of cell damage at this time point.

2009-09-15 | E-GEOD-14986 | biostudies-arrayexpress

Genomics of the ovine fetal brain ontogeny during the last stage of gestation

Project description:Normal development in the brain requires temporal changes in gene expression. A better understanding of the genomics of the fetal brain during late gestation can help to improve our understanding of the molecular events that enable the newborn to survive extra-uterine life. The purpose of the present study is to model changes and coherence of gene expression in cerebral cortex, brainstem, hippocampus, and hypothalamus throughout the second half of gestation. We used the sheep as an animal model to identify co-expressed genes in different regions of the ovine fetal brain, from mid-gestation (80 days) to one day of postnatal life. In the ewe, gestation length varies from 142 to 152 days, with an average of 147 days. Using a newly-available ovine array and weighted gene co-expression network analysis (WGCNA), we tested the hypothesis that the resulting products of genes expressed in a similar pattern through the last stage of gestation in different brain regions are functionally related and could play an important role in the normal fetal development. Tissues from cortex, brainstem, hippocampus and hypothalamus were collected from non treated fetuses at 80 (80d, n=4), 96M-bM-^@M-^S100 (100d, n=4), 120 (120d, n=4), 130 (130d, n=4), and 142M-bM-^@M-^S144 (145d, n=4) days of gestation and on the first (1d, n=4) day after delivery. Each group included one set of twin fetuses. None of the ewes showed any signs of impending labor.

2014-06-30 | E-GEOD-50460 | biostudies-arrayexpress

Transcription profiling of rat efferent ductules treated with the anti-estrogen fulvestrant ICI 182780

Project description:The aim of this study was to analyze the effects of the antiestrogen fulvestrant (ICI 182,780) on gene expression of the rat efferent ductules. Thirty day-old rats were treated once a week for 2 months with vehicle (control group) or ICI 182,780 (AstraZeneca; 10 m g/rat, s.c.). Total RNA of the efferent ductules was extracted using the RNeasy Mini Kit (QIAgen) an further treated with DNAse (QIAgen). Two ug of total RNA were used in the CodeLink Rat Whole Genome Bioarray (GE, Piscataway, NJ), according to the instructions of the manufacturer. The initial analysis was performed with the CodeLink Expression Analysis v4.0 Software (GE). Only genes with at least a 2-fold increase or decrease in expression were selected for further analysis. Statistical analysis was performed with the Significance Analysis of Microarray Program (SAM package).

2008-06-09 | E-MEXP-1497 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data