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The functional enhancer repertoire of human embryonic stem cells [isolated plasmid DNA-seq]

ABSTRACT: We combined Self-Transcribing Active Regulatory Region Sequencing (STARR-seq) with an enrichment step using chromatin immunoprecipitation in a massively parallel reporter assay. We applied this assay, termed ChIP-STARR-seq, to normal (primed) and naive human embryonic stem cells, building up a comprehensive catalogue of functional enhancers. This database record describes the DNA-seq component from isolated plasmids.

ORGANISM(S): Homo sapiens

PROVIDER: GSE99628 | GEO | 2017/07/05

SECONDARY ACCESSION(S): PRJNA389115

REPOSITORIES: GEO

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The functional enhancer repertoire of human embryonic stem cells [plasmid DNA-seq]

Project description:We combined Self-Transcribing Active Regulatory Region Sequencing (STARR-seq) with an enrichment step using chromatin immunoprecipitation in a massively parallel reporter assay. We applied this assay, termed ChIP-STARR-seq, to normal (primed) and naive human embryonic stem cells, building up a comprehensive catalogue of functional enhancers. This database record describes the DNA-seq component from plasmid libraries prior to transfection.

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ATAC-STARR-seq reveals transcription factor-bound activators and silencers across the chromatin accessible human genome

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