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Aplysia Pleural Ganglia 7 Days After Long-Term Sensitization Training

ABSTRACT: We used a custom-designed microarray and qPCR to characterize the transcriptional response that persists 7 days after long-term sensitization training in the marine mollusk Aplysia californica, a time-point at which recall has decayed but savings memory is still possible to induce. Aplysia were exposed to a 1-day unilateral LTS training protocol (4 rounds of noxious shock at 30 min intervals, each shock 10s of 1/2s off 1/2on 90mA 60-hz biphasic square-wave shock). Training produced robust sensitization in all animals (measured as an increase in T-SWR response from baseline to 24h after training). One week (7 days) after training, animals were again post-tested, and showed little-to-no evidence of recall (average change from baseline = -4% on the trained side, -4% on the untrained side). Pleural ganglia were then harvested. Samples from a left-trained and right-trained animal were combined to smooth out lateralized gene expression. The pleural ganglia contains the VC nociceptors, which are thought to play a primary role in expressing LTS memory.

ORGANISM(S): Aplysia californica

PROVIDER: GSE99792 | GEO | 2017/06/08

SECONDARY ACCESSION(S): PRJNA389603

REPOSITORIES: GEO

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