Project description:DDA non-targeted LC-MS/MS, PPL-SPE extracted marine organic matter. Positive Mode. Samples taken from during the Scripps Pier Diel Project 2022.
Project description:Acidified C18 and acidified and untreated PPL SPE extracted DOM samples taken during a 9-day study off the Scripps Pier in La Jolla (Non-targeted LC-MS/MS, positive mode).
Project description:Samples of marine DOM from the Ellen Browning Scripps Memorial Pier (3252001.500N 11715026.900W) in La Jolla, Southern Califiornia, USA, analyzed by offline 2D-LC-MS/MS
Project description:DDA non-targeted LC-MS/MS, PPL-SPE extracted marine organic matter. Positive Mode. Samples taken from during the Scripps Pier Diel Project 2022.
Project description:Acidified and untreated PPL SPE extracted DOM samples taken before during and after Hurricane Hillary 2023 at the Scripps Pier in La Jolla, CA (Non-targeted LC-MS/MS, positive and negative mode)
Project description:Non-targeted Metabolomics from PPL extracts from Seaspray Aerosol, River and Ocean DOM from the Tijuna Esturay, Imperial Beach and Scripps Pier. Collected Jan-Feb-March-May 2019.
Project description:Diazotrophs provide the main source of reactive nitrogen to the ocean, sustaining primary productivity and CO2 uptake. Climate change is raising temperatures, decreasing pH and reducing nutrient availability. How microbes respond to these changes is largely unexplained. Similarly, the role of DOM in the growth and survival of certain diazotrophic organisms is poorly understood. Moreover, growing evidence indicates some diazotrophs are capable of utilizing distinct DOM compounds via osmotrophy providing them with additional metabolic plasticity and ecological advantages compared to other non-diazotrophic microbes. We aimed to understand how osmotrophy could modify carbon uptake and alleviate energy stress in diazotrophs under ongoing climate change perturbations. We hypothesized that Crocosphaera preferentially uses DOM when labile as a carbon source in present pH conditions, as compared to future more acidic scenarios with higher access to inorganic carbon. Alternatively, the lower pH may cause Crocosphaera to be energy limited when trying to maintain intracellular homeostasis which would favour DOM uptake as an extra source of energy.