Project description:A co-culture of 60% GFP-labeled human mesenchymal stem cells (MSCGFP) and 40% mcherry-labeled NIH:OVCAR-3 tumor cells were incubated in MSC culture medium for up to 7 days in cell culture plates (diameter 10 cm, Greiner BioOne GmbH, Frickenhausen, Germany) at an initial density of 2,000 cells/cm2.
Project description:NIH-3T3 cells were infected with wildtype MCMV at a multiplicity of infection (MOI) of 10. Ribosome profiling was performed at the indicated times of infection as described in Rutkowski et al., Nature Communications 2015.