Project description:Plasma proteomics has regained attention in recent years through advancements in mass spectrometry instrumentation and sample preparation, as well as new high-throughput affinity-based technologies. Here, we evaluate the analytical performance of the new Olink Reveal platform, a proximity extension assay based technology quantifying 1,034 proteins across biological pathways. Using spiked-in recombinant Interleukin-10 (IL-10) and vascular endothelial growth factor D (VEGF-D) in the NIST SRM 1950 plasma standard, we assessed the linearity, sensitivity, precision and accuracy of the Olink assay. The results demonstrated strong linear relationships (R² 0.922–0.953) for both IL-10 and VEGF-D across spiked-in concentrations, confirming the robust technical performance of Olink Reveal and underscoring its suitability for relative quantitation in large-scale studies. The resulting data contains no sensitive or personally identifiable information, and is available in public repositories, and therefore suitable for use in benchmarking and software development.
Project description:Lipids in the reference material NIST 1950 (50 uL) were extracted accodring to the Matyash protocol. The sample was analysed in 5 technical replicates by ESI(-)-HILIC-TIMS-MS with PASEF enabled with 100 ms and 500 ms.
Project description:Lipids in the reference material NIST SRM 1950 (50 uL) were extracted according to the Matyash protocol. The sample was analysed in 5 technical replicates by ESI(-)-HILIC-TIMS-MS with PASEF enabled with 100 ms.
Project description:GNPS - NIST 1950 Serum SRM serial dilution processed with MZmine2 and OpenMS for Feature Based Molecular Networking. Files were obtained from the MSV000081364 (GNPS - NIST Standard Reference Material Human Serum - Column Comparability) https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=010ce4f32b1d4c56aff9402d370d0c15 NIST SRM-1950 was processed using an 80% EtOH extraction protocol (found here 10.1021/ac402503m) and subsequently ran on a UHPLC using a Phenomenex Luna 1.6u C18 Polar Data coupled to Q Exactive Orbtirap (Thermo Fisher Scientific). Files were converted from mzXML to mzML with MSConvert.