Project description:As the importance of transcriptional variation and regulation for Plasmodium becomes more apparent, advances for non-falciparum species are hindered by our reliance on natural infections to study parasite biology. Untargeted transcriptomic research is also complicated by low parasite densities and high proportions of human genetic material, highlighting the need for optimized sample processing protocols. In this study, we used a P. knowlesi culture diluted in whole blood as a mock P. vivax natural infection to compare white blood cell, rRNA-, and globin depletion methods and RNA-seq library preparation kits to create an optimized protocol for low-volume sample processing.
Project description:Untargeted Temporal Analysis of Serum using FPSE after 8 Gy radiation
Time points of room temperature serum analyzed: 1, 3, 24, 72, and 120 hours.
Project description:The aim of this study was to discover significantly changed proteins in human blood serum as a result of 6 h sleep loss at night. Eight females were reqruited. . Peripheral venous whole blood sampling was performed at 04:00, after 6 h of sleep and after 6 h of sleep deprivation (SD). Serum from each subject was depleted before protein digestion by trypsin and iTRAQ labeling. Labled peptides were analyzed by mass spectrometry.
Project description:Untargeted Temporal Analysis of Serum using FPSE after 8 Gy radiation
Time points of room temperature serum analyzed: 1, 3, 24, 72, and 120 hours.
Project description:Small molecule-based chemical reprogramming provides a fundamentally innovative approach for generating human chemically induced pluripotent stem cell. Our recent work has successfully applied this chemical strategy to efficiently reprogram human blood cells to pluripotency. However, the absence of serum-free conditions for blood cell reprogramming significantly limited the further clinical translation. Here, we established a robust and defined serum-free condition that enable the stable reprogramming of human blood cells into hCiPS cells. This method has been reliably reproduced across over 10 different donors and can generate hCiPS cells within 20 days. The serum-free blood derived-hCiPS cells exhibit typical characteristics of human pluripotent stem cells. Furthermore, this approach allows for efficient generation of hCiPS cells even from a small volume of fingerstick blood. In summary, this study established a powerful and defined conditions for deriving hCiPS cells from blood cells, thereby greatly facilitating their further clinical applications in regenerative medicine.