Project description:Top-Down proteomics pilot experiment of unfractionated Bovine Heart Mitochondria (BHM) using ultra high resolution Q-ToF tandem mass spectrometry (maXis 4G ETD, Bruker Daltonics).
Project description:Samples were processed on a Bruker Daltonics maXis Impact and C18 RP-UPLC for untargeted metabolomic analysis. Subset of MSV000083759 and MSV000083758.
Project description:Example metabolite analysis of food and stool samples. Samples were extracted in ethanol using FoodOmics protocols. Data were acquired using a Bruker Daltonics maXis Impact and C18 RP-UHPLC. Positive polarity acquisition of LC-MS and LC-MS/MS.
Project description:36 Uni-Cyanobacterial samples containing associated heterotrophs collected via UltiMate 3000 UHPLC system (Thermo Scientific) using a Kinetex 1.7 micrometers C18 reversed phase UHPLC column (50 x 2.1 mm) and Maxis Impact Q-TOF mass spectrometer (Bruker Daltonics) equipped with an ESI source.
Project description:Cyanobacteria extracts and SPE fractions. LC MSMS analysis was performed in an UltiMate 3000 UPLC system (Thermo Scientific) using a Kinetex 1.7 mm C18 reversed phase UHPLC column (50 X 2.1 mm) and Maxis Impact HD Q-TOF mass spectrometer (Bruker Daltonics) equipped with ESI source.
Project description:The present study was initiated to evaluate the quantitative proteomic profiling of Protothecazopfiigenotypes. The cells (P.zopfii genotype 1 –noninfectious and genotype 2 -infectious) were cultured in triplicates until the mid-logarithmic growth phase; the proteins were extracted after sonication on ice, followed by 2D DIGE separation as recommended by the manufacturer (GE Healthcare). The differentially expressed proteins spots were identified using Decodon software analysis (Delta 2D version 4.0 software). Protein identification was carried out by MALDI TOF MS/MS (Ultraflex III TOF/TOF, Bruker Daltonics, Bremen, Germany). The spectra was acquired using the automated option (AutoXecute ) of the Flex Analysis software version 3.3 (Bruker Daltonics, Leipzig, Germany), processed using Flex analysis software version 3.3 (Bruker Daltonics, Leipzig, Germany) and the database search was conducted using the MS/MS ion search (MASCOT, http://www.matrixscience.com) against all entries of NCBInr (GenBank)/Swissprot with subsequent parameters: trypsin digestion, up to one missed cleavage, fixed modifications: carbamidomethyl and with the following variable modifications: oxidation (M), peptide tol.: +- 1.2 Da, MS/MS tol.: +- 0.6 Da, peptide charge: +1. The results were assessed using MOWSE score, p- and E values and those possess positive hits with cRAP database were eliminated from the list.
Project description:Human breast milk samples were extracted with ethanol and processed on a Bruker Daltonics maXis Impact and C18 RP-UPLC for untargeted metabolomic analysis. Positive polarity acquisition of LC-MS/MS.
Project description:Subset of AGP fecal samples. Samples were extracted with ethanol and processed on a Bruker Daltonics maXis Impact and C18 RP-UPLC for untargeted metabolomic analysis. Positive polarity acquisition of LC-MS/MS.
Project description:Late preterm infant samples from multiple body sites. Samples were extracted with ethanol and processed on a Bruker Daltonics maXis Impact and C18 RP-UPLC for untargeted metabolomic analysis. Positive polarity acquisition of LC-MS/MS.