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MetaboLights MTBLS567 - GNPS Intracellular and extracellular metabolites from three strains of Prochlorococcus

ABSTRACT: Prochlorococcus is an abundant, cosmopolitan, marine cyanobacterium with ecotypes that vary temporally and spatially across oligotrophic regions of the global ocean. This group of organisms can serve as a model system to understand the accumulation of organic compounds synthesized by primary producers in marine ecosystems. We applied targeted metabolomics to three axenic cultures of strains that span the Prochlorococcus phylogeny: a high-light adapted HLII-clade strain, a low-light adapted LLI-clade strain, and a low-light adapted LLIV-clade strain. Intracellular metabolites were extracted from cells captured in exponential growth and extracellular metabolites were adsorbed, from the same samples, to solid-phase extraction resin. Both pools were quantified using a triple quadrupole mass spectrometer. The resulting data reveal intraspecific differences in metabolites that provide clues about the selective pressures shaping the meta-metabolism of the Prochlorococcus collective, and its interactions with the surrounding microbes that depend on them.

INSTRUMENT(S): Thermo Scientific instrument model|1000494

ORGANISM(S): Prochlorococcus|1218

SUBMITTER: Krista Longnecker

PROVIDER: MSV000085790 | GNPS | Mon Jul 20 16:07:00 BST 2020

REPOSITORIES: GNPS

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Project description:Rex2013 - Genome scale metabolic model of D.shibae (iDsh827) The aerobic anoxygenic phototroph Dinoroseobacter shibae DFL12T is a representative of an important group of marine bacteria called the Roseobacter clade. To gain insight into the versatile metabolism of this clade, the authors have taken a constraint-based approach and created this genome-scale metabolic model (iDsh827). This model is the first one to account for the energy demand of motility, the light-driven ATP generation and experimentally determined specific biomass composition. This model is described in the article: Swimming in light: a large-scale computational analysis of the metabolism of Dinoroseobacter shibae. Rex R, Bill N, Schmidt-Hohagen K, Schomburg D. PLoS Comput Biol. 2013;9(10):e1003224. Abstract: The Roseobacter clade is a ubiquitous group of marine α-proteobacteria. To gain insight into the versatile metabolism of this clade, we took a constraint-based approach and created a genome-scale metabolic model (iDsh827) of Dinoroseobacter shibae DFL12T. Our model is the first accounting for the energy demand of motility, the light-driven ATP generation and experimentally determined specific biomass composition. To cover a large variety of environmental conditions, as well as plasmid and single gene knock-out mutants, we simulated 391,560 different physiological states using flux balance analysis. We analyzed our results with regard to energy metabolism, validated them experimentally, and revealed a pronounced metabolic response to the availability of light. Furthermore, we introduced the energy demand of motility as an important parameter in genome-scale metabolic models. The results of our simulations also gave insight into the changing usage of the two degradation routes for dimethylsulfoniopropionate, an abundant compound in the ocean. A side product of dimethylsulfoniopropionate degradation is dimethyl sulfide, which seeds cloud formation and thus enhances the reflection of sunlight. By our exhaustive simulations, we were able to identify single-gene knock-out mutants, which show an increased production of dimethyl sulfide. In addition to the single-gene knock-out simulations we studied the effect of plasmid loss on the metabolism. Moreover, we explored the possible use of a functioning phosphofructokinase for D. shibae. This model is hosted on BioModels Database and identified by: MODEL1308180000 . To cite BioModels Database, please use: BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models . To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information.

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MetaboLights MTBLS567 - GNPS Intracellular and extracellular metabolites from three strains of Prochlorococcus

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