MetaboLights MTBLS746 - GNPS Spatial metabolomics of in situ, host-microbe interactions (UPLC-MS/MS assay)

ABSTRACT: Spatial metabolomics describes the location and chemistry of small molecules involved in metabolic phenotypes, defense molecules and chemical interactions in natural communities. Most current techniques are unable to spatially link the genotype and metabolic phenotype of microorganisms in situ at a scale relevant to microbial interactions. Here, we present a spatial metabolomics pipeline (metaFISH) that combines fluorescence in situ hybridization (FISH) microscopy and high-resolution atmospheric pressure mass spectrometry imaging (AP-MALDI-MSI) to image host-microbe symbioses and their metabolic interactions. metaFISH aligns and integrates metabolite and fluorescent images at the micrometer-scale for a spatial assignment of host and symbiont metabolites on the same tissue section. To illustrate the advantages of metaFISH, we mapped the spatial metabolome of a deep-sea mussel and its intracellular symbiotic bacteria at the scale of individual epithelial host cells. Our analytical pipeline revealed metabolic adaptations of the epithelial cells to the intracellular symbionts, a variation in metabolic phenotypes in one symbiont type, and novel symbiosis metabolites. metaFISH provides a culture-independent approach to link metabolic phenotypes to community members in situ - a powerful tool for microbiologists across fields.

UPLC-MS/MS assay protocols and data are reported in the current study MTBLS746.

MALDI imaging MS assay protocols and data associated to this study are reported in MTBLS744.
On-tissue MALDI imaging MS/MS assay protocols and data associated to this study are reported in MTBLS805.
MRMS assay protocols and data associated to this study are reported in MTBLS811.

INSTRUMENT(S): Q Exactive|1001911

ORGANISM(S): Bathymodiolus Antarcticus|1684310

SUBMITTER: Benedikt Geier  

PROVIDER: MSV000085882 | GNPS | Sat Aug 01 14:08:00 BST 2020

REPOSITORIES: GNPS