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GNPS Metabolomics of plant pathogen Streptomyces sp. 11-1-2

ABSTRACT: Metabolomics profile of Streptomyces sp. 11-1-2, a plant pathogen isolated from common scab lesions on potato in Newfoundland, Canada. Positive and negative ionisation mode. The deposition contains RAW and mzXML.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Streptomyces Sp. 11-1-2 (ncbitaxon:1851167)

SUBMITTER: Dawn R. D. Bignell

PROVIDER: MSV000086628 | GNPS | Mon Dec 21 18:23:00 GMT 2020

REPOSITORIES: GNPS

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Nigericin and Geldanamycin Are Phytotoxic Specialized Metabolites Produced by the Plant Pathogen Streptomyces sp. 11-1-2.

Díaz-Cruz Gustavo A GA Liu Jingyu J Tahlan Kapil K Bignell Dawn R D DRD

Microbiology spectrum 20220228 2

Streptomyces bacteria are a key source of microbial specialized metabolites with useful applications in medicine and agriculture. In addition, some species are important plant pathogens and cause diseases such as potato scab, which reduces the quality and market value of affected potato crops. Most scab-associated Streptomyces spp. produce the phytotoxic metabolite thaxtomin A as the principal pathogenicity factor. However, recent reports have described scab-causing strains that do ...[more]

PMID: 35225656

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Metabolomics of plant pathogen Streptomyces sp. 11-1-2

Project description:Metabolomics profile of Streptomyces sp. 11-1-2, a plant pathogen isolated from common scab lesions on potato in Newfoundland, Canada. Positive and negative ionisation mode. The deposition contains RAW and mzXML.

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Role of oxylipins in pathogen defense of potato

Project description:Oxylipins play a role in the response of plants to pathogens both as antimicrobial compounds and as signaling molecules. In potato, pathogen infection leads to the stimulation of the 9-lipoxygenase pathway (Göbel et al. 2001; 2002; Stumpe et al. 2001). In order to analyze whether 9-LOX-derived oxylipins such as colnele(n)ic acid (products of the 9-divinyl ether synthase reaction) act as signaling molecules for the activation of defense responses, transgenic potato plants (Solanum tuberosum cv. Désirée) were generated which express an RNAi construct directed against the pathogen-induced 9-lipoxygenase (Göbel et al. 2003) or against the pathogen-induced 9-divinyl ether synthase (Stumpe et al. 2001). Highly reduced transcript levels correlate with low levels of 9-lipoxygenase-derived oxylipins (Göbel et al. 2003). Transgenic and wild type plants were grown as sterile plants on MS medium supplemented with agar in a phytochamber with 16 h of light [200 µE] at 22 °C. After transfer to soil, plants were kept in a phytochamber with 16 h of light [200 µE], 18 °C and 60 % humidity for four weeks. Lower leaves were infiltrated with a suspension of Pseudomonas syringae pv. maculicola at a concentration of 108 cfu/ml MgCl2 or, as a control, 10 mM MgCl2-solution. RNA was isolated both from the infiltrated leaves and the upper, non-treated leaves using the trizol method. Subsequently, RNA was digested with DNase and purified via Qiagen RNeasy Kit (Qiagen, Hilden, Germany) according to the manufacturer´s instructions. Keywords: Direct comparison

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Halorubrum sp. 11 strain:11

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Microarray-based species identification of Streptomyces spp. isolated from potato scab lesions in Norway

Project description:28 Streptomyces strains isolated from common scab lesions of potato tubers from a wide geographic range in Norway, were selected for microarray analysis. The selected strains were subjected to species identification by microarray, 16S phylogenetic analysis and PCR; and microarray-based comparative genome analysis. To our knowledge, this is the first report of S. turgidiscabies and S. europaeiscabiei in Norway.

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Gene expression data of QRsP-11 mouse fibrosarcoma cell line and its liver metastatic subline

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