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GNPS - Artificial Sputum Medium Formulation Impacts Psuedomonas aeruginosa Chemotype

ABSTRACT: Paper Title: Artificial Sputum Medium Formulation, Including Porcine Gastric Mucin, Impacts Pseudomonas aeruginosa Phenotype and Chemotype Author List: Neve RL and Phelan VV. Brief description of data submitted: Metabolomics results of PAO1 grown in 9 ASM and LB medium. Raw data, open source data, feature finding output files, and Cytoscape network files used to generate Figures 2 Classical Molecular Network and 3 Feature Based Molecular Network.

INSTRUMENT(S): impact

ORGANISM(S): Pseudomonas Aeruginosa Pao1 (ncbitaxon:208964)

SUBMITTER: Vanessa V. Phelan

PROVIDER: MSV000086721 | GNPS | Tue Jan 19 15:14:00 GMT 2021

REPOSITORIES: GNPS

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Project description:Transcription factor Foxq1 controls mucin gene expression and granule content in mouse stomach surface mucous cells ; Background and Aims: The gastric mucosa provides a stringent epithelial barrier and produces acid and enzymes that initiate digestion. In this regenerating tissue, progenitors differentiate continually into 4 principal specialized cell types, yet underlying mechanisms of differentiation are poorly understood. We identified stomach-restricted expression of the forkhead transcription factor FOXQ1. Methods: We used a combination of genetic, histochemical, ultrastructural and molecular analysis to study gastric cell lineages with respect to FOXQ1. Results: Within the developing and adult gastrointestinal tract, Foxq1 mRNA is restricted to the stomach, expressed prominently in foveolar (pit) cells, the abundant mucin-producing cells that line the mucosal surface, and required for their complete differentiation. Mice carrying Foxq1 coding mutations show virtual absence of mRNA and protein for the backbone of the predominant stomach mucin, MUC5AC. These observations correspond to a paucity of foveolar-cell secretory vesicles and notable loss of stomach but not intestinal mucus. Transcriptional profiling identified a surprisingly restricted set of genes with altered expression in Foxq1 mutant stomachs. MUC5AC is a highly tissue-restricted product that similarly depends on FOXQ1 in its other major site of expression, conjunctival goblet cells. Conclusions: Taken together, these observations imply that promotion of gastric MUC5AC synthesis is a primary, cell-autonomous function of FOXQ1. This study is the first to implicate a transcription factor in terminal differentiation of foveolar cells and begins to define the requirements to assemble highly specialized organelles and cells in the gastric mucosa. DOI: 10.1053/j.gastro.2008.04.019 Experiment Overall Design: RNA from stomach antrum from 2 of each Satin, Beige, and BL6 mice were compared using microarray analysis.

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GNPS - Artificial Sputum Medium Formulation Impacts Psuedomonas aeruginosa Chemotype

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