Project description:Comparison of LeuEnk, enolase, and HeLa tryptic peptides MS/MS spectra obtained on a Bruker QTof CID and a Thermo Q-Exactive Focus Orbitrap HCD instrument as a function of collision energy using the similarity index.
Project description:Various evolutionary trajectories lead to loss of activity of tobramycin-potentiating compounds in Burkholderia cenocepacia biofilms
Project description:Severe acute hypoxic stress is a major contributor to the pathology of human diseases, including ischemic disorders. Current treatments focus on managing consequences of hypoxia, with few addressing cellular adaptation to low-oxygen environments. Here, we hypothesize that accelerating hypoxia adaptation could provide a strategy to alleviate acute hypoxic stress. We developed a high-content phenotypic screening platform to identify compounds that fast-track adaptation to hypoxic stress. Our platform captures a high-dimensional phenotypic hypoxia response trajectory consisting of normoxic, acutely stressed, and chronically adapted cell states. Leveraging this trajectory, we identify compounds that phenotypically shift cells from the acutely stressed towards the adapted state, revealing mTOR/PI3K or BET inhibition as strategies to induce this phenotypic shift. Importantly, our compound hits promote the survival of liver cells exposed to ischemia-like stress, and rescue cardiomyocytes from hypoxic stress. Our platform offers a general, target-agnostic strategy to accelerate cellular adaptation, applicable across various stress conditions.
Project description:Examination of the impact of vitamin B12 and various one-carbon metabolism and/or epigenetic modifieres during in vitro OSKM reprogramming