Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

GNPS Outdoor versus indoor cultivation: effects on the metabolite profile of Agaricus subrufescens strains analyzed by untargeted metabolomics

ABSTRACT: Five commercial strains of the culinary and medicinal mushroom Agaricus subrufescens were grown under two cultivation systems (outdoor versus indoor). Polar and lipid extracts of mushroom dry powders were extracted with a mixture of solvents (water:methanol:methyl tert-buthyl ether) and analyzed by UHPLC-ESI-HRMS-based untargeted metabolomics. A Nexera X2 UHPLC system (Shimadzu) equipped with an Acquity UPLC HSS T3 column (2.1 mm x 100 mm x 1.8 um) (Waters) was employed coupled to a MaXis 4G Q-TOF MS analyzer (Bruker Daltonics) through an electrospray ionization source. Data was acquired in positive ion mode.

INSTRUMENT(S): maXis 4G

ORGANISM(S): Agaricus Subrufescens (ncbitaxon:87252)

SUBMITTER: Caio de Oliveira Gorgulho Silva

PROVIDER: MSV000087377 | GNPS | Thu May 06 13:29:00 BST 2021

REPOSITORIES: GNPS

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Outdoor versus indoor cultivation: effects on the metabolite profile of Agaricus subrufescens strains analyzed by untargeted metabolomics

Project description:Five commercial strains of the culinary and medicinal mushroom Agaricus subrufescens were grown under two cultivation systems (outdoor versus indoor). Polar and lipid extracts of mushroom dry powders were extracted with a mixture of solvents (water:methanol:methyl tert-buthyl ether) and analyzed by UHPLC-ESI-HRMS-based untargeted metabolomics. A Nexera X2 UHPLC system (Shimadzu) equipped with an Acquity UPLC HSS T3 column (2.1 mm x 100 mm x 1.8 um) (Waters) was employed coupled to a MaXis 4G Q-TOF MS analyzer (Bruker Daltonics) through an electrospray ionization source. Data was acquired in positive ion mode.

2021-05-06 | MSV000087377 | MassIVE

The physiology of Agaricus bisporus in semi-commercial compost cultivation appears to be highly conserved among unrelated isolates

Project description:The white button mushroom Agaricus bisporus is the most widely produced edible fungus with a great economical value. Its commercial cultivation process is often performed on wheat straw and animal manure based compost that mainly contains lignocellulosic material as a source of carbon and nutrients for the mushroom production. As a large portion of compost carbohydrates are left unused in the current mushroom cultivation process, the aim of this work was to study wild-type A. bisporus strains for their potential to convert the components that are poorly utilized by the commercial strain A15. Growth profiling suggested different abilities for several A. bisporus strains to use plant biomass derived polysaccharides, as well as to transport and metabolize the corresponding monomeric sugars. Six wild-type isolates with diverse growth profiles were compared for mushroom production to A15 strain in semi-commercial cultivation conditions. Transcriptome and proteome analyses of the three most interesting wild-type strains and A15 indicated that the unrelated A. bisporus strains degrade and convert plant biomass polymers in a highly similar manner. This was also supported by the chemical content of the compost during the mushroom production process. Our study therefore reveals a highly conserved physiology for unrelated strains of this species during growth in compost.

2018-05-22 | GSE99928 | GEO

Saccharomyces cerevisiae

Project description:Formaldehyde treatment with 1.8 mM for 2 h

| PRJNA102831 | ENA

MUSHROOM CULTIVATION

Project description:Bacterial-fungal interaction in mushroom cultivation

| PRJNA589209 | ENA

GNPS - Komagataella_phaffii_metabolites_RP_MeOH

Project description:LC-MS/MS metabolomics data of Komagataella phaffii recorded on a Q Exactive HF in positive and negative ion mode. Acquity HSS T3 (2.1 mm x 150 mm, 1.8 um, Waters) reverse phase column A: H2O 0.1% FA, B: 100% MeOH

2022-08-01 | MSV000090053 | GNPS

Proteomics analysis of Hypsizygus marmoreus

Project description:Hypsizygus marmoreus is one of the most popular industrialized cultivation mushrooms in East Asia. H. marmoreus is the third wildly cultivated industrialized cultivated mushroom in China now behind only Flammulina velutipes and Pleurotus eryngii. H. marmoreus is a low-temperature fruiting mushroom. The mycelia grow vegetatively at 23 °C to 25 °C and transform into fruiting process at 13 °C to 17 °C. The fruiting process of H. marmoreus is sensitive to temperature, and high temperature can lead to the death of mycelia and malformation of the fruiting body. Therefore, thermotolerance is important for H. marmoreus during both vegetative growth and fruiting-body formation. Previous studies on H. marmoreus have mainly focused on cultivation, nutrition and bioactive compounds extracted, but little is known about thermotolerance. The aim of this project is to elucidate the thermotolerance mechanism of Hypsizygus marmoreus.

2020-12-14 | PXD017654 | Pride

Single cell gene expression in Dlxi1/2b::eGFP+ cells before and after migration

Project description:We report the expression of selected genes associated with cortical, striatal and olfactory interneurons in hSS-derived Dlxi1/2::eGFP cells before (in hSS) and after migration (in hCS). By fusing hiPSC-derived fusions that were separately patterned to generate either human subpallial spheroids (hSS) or human cortical spheroids (hCS) we are able to model human interneuron migration in vitro. The aim of this experiment is to examine the gene expression of hSS-derived Dlxi1/2::eGFP cells before and after migration.

2017-04-26 | GSE96045 | GEO

Single cell gene expression in Dlxi1/2b::eGFP+ cells before and after migration, 4 weeks

2017-04-26 | GSE95837 | GEO

Single cell gene expression in Dlxi1/2b::eGFP+ cells before and after migration, 2 weeks

2017-04-26 | GSE93321 | GEO

GNPS - Komagataella_phaffii_metabolites_RP_ACN

2022-07-29 | MSV000090012 | GNPS