Dataset Information

GNPS - C. elegans as a model for inter-individual variation in metabolism

ABSTRACT: Fox BW, Ponomarova O, Lee YU, Zhang G, Giese EG, Walker M, Roberto NM, Na H, Reis-Rodrigues P, Curtis BJ, Kolodziej AR, Crombie TA, Zdraljevic S, Yilmaz LS, Andersen EC, Schroeder FC, Walhout AJM (2022) C. elegans as a model for inter-individual variation in metabolism

INSTRUMENT(S): Q Exactive HF, Q Exactive

ORGANISM(S): Caenorhabditis Elegans (ncbitaxon:6239)

SUBMITTER: Frank Schroeder

PROVIDER: MSV000087810 | GNPS | Tue Jul 13 11:43:00 BST 2021

REPOSITORIES: GNPS

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Publications

C. elegans as a model for inter-individual variation in metabolism.

Fox Bennett W BW Ponomarova Olga O Lee Yong-Uk YU Zhang Gaotian G Giese Gabrielle E GE Walker Melissa M Roberto Nicole M NM Na Huimin H Rodrigues Pedro R PR Curtis Brian J BJ Kolodziej Aiden R AR Crombie Timothy A TA Zdraljevic Stefan S Yilmaz L Safak LS Andersen Erik C EC Schroeder Frank C FC Walhout Albertha J M AJM

Nature 20220706 7919

Individuals can exhibit differences in metabolism that are caused by the interplay of genetic background, nutritional input, microbiota and other environmental factors<sup>1-4</sup>. It is difficult to connect differences in metabolism to genomic variation and derive underlying molecular mechanisms in humans, owing to differences in diet and lifestyle, among others. Here we use the nematode Caenorhabditis elegans as a model to study inter-individual variation in metabolism. By comparing three wi ...[more]

PMID: 35794472

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Project description:Study hypothesis: CRC risk is affected by diet and by genetic polymorphisms leading to inter-individual variation in the metabolism of environmental carcinogens. Our first hypothesis is that these effects may combine to place certain individuals at greatly enhanced risk according to their diet. We shall examine how dietary factors known to affect CRC risk (including meats, fat, fruit and vegetables, cooking methods, dietary supplements and drugs) and genetic polymorphisms implicated in CRC (Glutathione S-transferase: GSTM1, GSTM3, GSTT1; N-acetyl transferase: NAT1, NAT2; and Cytochrome P450: CYP1A1, CYP2D6) combine to affect the prevalence of colorectal polyps, both adenomatous (the precursor lesion of CRC) and metaplastic. Our second hypothesis is that diets associated with increased risk (high red meat, low vegetable and low fibre consumption) may affect DNA damage markers which may be elevated in people with colorectal adenomas. We shall therefore examine the effect of diet on adduct rates (cross sectional) and adduct rates in people with and without colorectal adenomas. We shall examine malondialdehyde adduct rates in relation to anti-oxidant status and carboxymethyl adduct rates and k-ras mutation frequency in relation to meat consumption; and examine the effect of DNA repair enzyme (ATase) activity on any relationship found. In summary the aim of this population based study is to link dietary factors thought to be important in the genesis of colorectal polyps and cancer with genetic polymorphisms and DNA damage markers in rectal mucosa and in blood. Primary outcome(s): We shall perform cross-sectional and case-control analyses of the effect of diet and genetic polymorphisms on the prevalence of adenomatous and metaplastic polyps. Genetic polymorphisms will be examined in isolation and paired combinations and in groups stratified according to diet. We shall examine the effect of diet on MDA adduct rates (concentrating on foods relating to anti-oxidant status) and CBM adduct rates and k-ras mutations (concentrating on meat consumption and cooking methods) by categorical variables; and MDA and CBM adducts and k-ras mutation frequency in people with adenomatous polyps and polyp free controls (case-control). We shall examine the affect of ATase activity on any relationship found and ATase activity in people with adenomatous polyps and polyp free controls (case control). DNA will be stored for future analysis of as yet unrecognised polymorphisms which may be recognised as having a role in CRC. Samples taken from EPIC participants for adduct, ATase and k-ras measurement whose polyps are found to be metaplastic will be stored for possible future analysis: we shall investigate people with adenomatous polyps in the first instance as these are the precursors of colorectal cancer.

Dataset Information

GNPS - C. elegans as a model for inter-individual variation in metabolism

Publications

C. elegans as a model for inter-individual variation in metabolism.

Similar Datasets

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