GNPS Mouse Brain Lipidomics_Vasilopoulou CG
Ontology highlight
ABSTRACT:
INSTRUMENT(S): timsTOF Pro
ORGANISM(S): Mus Musculus (ncbitaxon:10090)
SUBMITTER: 
Catherine G. Vasilopoulou
PROVIDER: MSV000088020 | GNPS | Wed Aug 25 01:43:00 BST 2021
REPOSITORIES: GNPS
Similar Datasets
Project description:Lipidome profiling of mouse brain tissue using a highly sensitive nanoLC ion mobility QTOF workflow
2021-08-25 | MSV000088020 | MassIVE
Project description:<p><strong>INTRODUCTION:</strong> The absolute quantitation of lipids at the lipidome-wide scale is a challenge but plays an important role in the comprehensive study of lipid metabolism. </p><p><strong>OBJECTIVES:</strong> We aim to develop a high-throughput quantitative lipidomics approach to enable the simultaneous identification and absolute quantification of hundreds of lipids in a single experiment. Then, we will systematically characterize lipidome-wide changes in the aging mouse brain and provide a link between aging and disordered lipid homeostasis. </p><p><strong>METHODS:</strong> We created an in-house lipid spectral library, containing 76,361 lipids and 181,300 MS/MS spectra in total, to support accurate lipid identification. Then, we developed a response factor-based approach for the large-scale absolute quantifications of lipids. </p><p><strong>RESULTS:</strong> Using the lipidomics approach, we absolutely quantified 1212 and 864 lipids in human cells and mouse brains, respectively. The quantification accuracy was validated using the traditional approach with a median relative error of 12.6%. We further characterized the lipidome-wide changes in aging mouse brains, and dramatic changes were observed in both glycerophospholipids and sphingolipids. Sphingolipids with longer acyl chains tend to accumulate in aging brains. Membrane-esterified fatty acids demonstrated diverse changes with aging, while most polyunsaturated fatty acids consistently decreased. </p><p><strong>CONCLUSION:</strong> We developed a high-throughput quantitative lipidomics approach and systematically characterized the lipidome-wide changes in aging mouse brains. The results proved a link between aging and disordered lipid homeostasis. </p><p><br></p><p><strong>Mouse brain NEG UPLC-MS assay</strong> data is reported in the current study <a href='https://www.ebi.ac.uk/metabolights/MTBLS495' rel='noopener noreferrer' target='_blank'><strong>MTBLS495</strong></a>.</p><p><strong>Mouse brain POS UPLC-MS assay</strong> data associated to this study is reported in <a href='https://www.ebi.ac.uk/metabolights/MTBLS562' rel='noopener noreferrer' target='_blank'><strong>MTBLS562</strong></a>.</p>
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Project description:<p><strong>INTRODUCTION:</strong> The absolute quantitation of lipids at the lipidome-wide scale is a challenge but plays an important role in the comprehensive study of lipid metabolism. </p><p><strong>OBJECTIVES:</strong> We aim to develop a high-throughput quantitative lipidomics approach to enable the simultaneous identification and absolute quantification of hundreds of lipids in a single experiment. Then, we will systematically characterize lipidome-wide changes in the aging mouse brain and provide a link between aging and disordered lipid homeostasis. </p><p><strong>METHODS:</strong> We created an in-house lipid spectral library, containing 76,361 lipids and 181,300 MS/MS spectra in total, to support accurate lipid identification. Then, we developed a response factor-based approach for the large-scale absolute quantifications of lipids. </p><p><strong>RESULTS:</strong> Using the lipidomics approach, we absolutely quantified 1212 and 864 lipids in human cells and mouse brains, respectively. The quantification accuracy was validated using the traditional approach with a median relative error of 12.6%. We further characterized the lipidome-wide changes in aging mouse brains, and dramatic changes were observed in both glycerophospholipids and sphingolipids. Sphingolipids with longer acyl chains tend to accumulate in aging brains. Membrane-esterified fatty acids demonstrated diverse changes with aging, while most polyunsaturated fatty acids consistently decreased. </p><p><strong>CONCLUSION:</strong> We developed a high-throughput quantitative lipidomics approach and systematically characterized the lipidome-wide changes in aging mouse brains. The results proved a link between aging and disordered lipid homeostasis. </p><p><br></p><p><strong>Mouse brain POS UPLC-MS assay</strong> data is reported in the current study <a href='https://www.ebi.ac.uk/metabolights/MTBLS562' rel='noopener noreferrer' target='_blank'><strong>MTBLS562</strong></a>. </p><p><strong>Mouse brain NEG UPLC-MS assay</strong> data associated to this study is reported in <a href='https://www.ebi.ac.uk/metabolights/MTBLS495' rel='noopener noreferrer' target='_blank'><strong>MTBLS495</strong></a>.</p>
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