Project description:Microbes were isolated from frass material collected within laboratory galleries of Odontotaenius disjunctus (bessbug beetles) in the USA, and cultured on ISP2-agar for seven days at 30C. Cultures were extracted with ethyl acetate or methanol.
Project description:Three independent cultures of Methylorubrum extorquens PA1 delta cel were grown on ammonium mineral salts with either methanol or succinate provided as the sole carbon and energy source. The supernatant was subsequently extracted with acidified ethyl acetate and analyzed by LC-MS.
Project description:Clostridium beijerinckii is an anaerobic strain and well known for acetone-ethanol-butanol (ABE) fermentation using carbohydrates derived from cellulose or starch. During ABE fermentation, various byproducts are formed, mainly including acids (acetate, butyrate and lactate) and gas (hydrogen and carbon dioxide). recently, we found that Clostridium beijerinckii is able to produce a new product that had never been reported before and tightly regulated by pH and nitrogen source.
Project description:Enrichment culture DFE is capable of fermenting the toxic pollutant dichloromethane (DCM; CH2Cl2) into environmentally benign acetate. The dominant organism in the enrichment culture is a novel bacterium in the Peptococcaceae family, “Candidatus Formimonas warabiya” strain DCMF. This metaproteomic study analysed culture DFE during growth with four substrates – DCM, glycine betaine, choline, and methanol – all of which are primarily, if not solely, metabolised by strain DCMF.
Project description:PKS14-overexpressing strain was inoculated into PDB for 7 days. The fungal cells were extracted with methanol, and the culture broth was extracted with ethyl acetate.
Project description:The PKS14-knockout strain was inoculated into PDB for 7 days. The fungal cells were extracted with methanol, and the culture broth was extracted with ethyl acetate.
Project description:Samples from monoculture of bacteria or fungi grown for 24h. Samples were extracted with methanol 80% or ethyl-acetate (supernatant fractions)