Project description:Non-targeted LC-MS/MS analysis of PPL solid phase extracted dissolved organic matter (DOM) from TARA/TREC Expedition Leg 1, collected in the coastal Atlantic between France to Netherlands in Spring 2023.
Project description:Optimization of Solid Phase Extraction Columns (C18, HBL, PPL) for non-targeted LC-MS/MS analysis of river dissolved organic matter.
Project description:Many trees form ectomycorrhizal symbiosis with fungi. During symbiosis, the tree roots supply sugar to the fungi in exchange for nitrogen, and this process is critical for the nitrogen and carbon cycles in forest ecosystems. However, the extents to which ectomycorrhizal fungi can liberate nitrogen and modify the soil organic matter and the mechanisms by which they do so remain unclear since they have lost many enzymes for litter decomposition that were present in their free-living, saprotrophic ancestors. Using time-series spectroscopy and transcriptomics, we examined the ability of two ectomycorrhizal fungi from two independently evolved ectomycorrhizal lineages to mobilize soil organic nitrogen. Both species oxidized the organic matter and accessed the organic nitrogen. The expression of those events was controlled by the availability of glucose and inorganic nitrogen. Despite those similarities, the decomposition mechanisms, including the type of genes involved as well as the patterns of their expression, differed markedly between the two species. Our results suggest that in agreement with their diverse evolutionary origins, ectomycorrhizal fungi use different decomposition mechanisms to access organic nitrogen entrapped in soil organic matter. The timing and magnitude of the expression of the decomposition activity can be controlled by the below-ground nitrogen quality and the above-ground carbon supply.
Project description:Effect of the contribution of nitrogen and dissolved organic matter derived from salmon farming on the bacterioplanctonic diversity in the town of Puyuhuapi, North Patagonia.