Project description:Glutamicibacter arilaitensis grown in large scale liquid Cheese Curd media and extracted with Amberlite XAD16, back extracted with MeOH:DCM 50:50, separated on SPE column to afford 7 fractions labeled A through G based on increasing polarity (A = 10% MeOH, F = 100% MeOH and G = 100% EtoAC. Fractions E(60% MeOH), D(80% MeOH), and F(100% MeOH) run on a qTOF and submitted here. suffix 1 refers to treatment of cultures with desferrioxamine and suffix PLAIN means untreated. JB182new_20170614 is a crude extract from a subsequent small scale growup without desferrioxamine.
Project description:Crude CHCl3-MeOH extract and n-hexane, CHCl3, and EtOAc fractions from leaves of Melicope pteleifolia collected in Vietnam. Subfractions obtained from n-hexane fraction are also included.
Project description:SPE of Synechococcus elongatus cell extract. SPE was performed using a C18 column. The samples are elution fractions of different MeOH concentrations (20,40,60,80,100 percent).
Project description:Glutamicibacter arilaitensis grown in large scale liquid Cheese Curd media and extracted with Amberlite XAD16, back extracted with MeOH:DCM 50:50, separated on SPE column to afford 7 fractions labeled A through G based on increasing polarity (A = 10% MeOH, F = 100% MeOH and G = 100% EtoAC. Fractions E(60% MeOH), D(80% MeOH), and F(100% MeOH) run on a qTOF and submitted here. suffix 1 refers to treatment of cultures with desferrioxamine and suffix PLAIN means untreated. JB182new_20170614 is a crude extract from a subsequent small scale growup without desferrioxamine.
Project description:45 samples consisting of 1:1 MeOH:H2O, and MeOH C18 RP-SPE fractions of mycelium and broth extracts of cultures of the amphibian gut-symbiotic fungus Basidiobolus meristosporus, prepared by Spatafora Lab and McPhail Lab.
Project description:The dataset contains the data of key experiments for developing a novel and highly sensitive bottom-up phosphoproteomics strategy termed ERLIC-SCX/RP-LC-MS. We compared two SPE types (RP, SCX) for an ERLIC run with 7 fractions. The results were used to design a workflow for highly sensitive bottom-up phosphoproteomics (third experiment) combing ERLIC in 21 fractions with the fractions 1-9 cleaned by SCX-SPE and the fractions 10-21 by RP-SPE. Up to 50% of the fractions were subjected to LC-MS/MS analysis with 45 h of total MS time per replicate.
Project description:During senescence of detached rice leaves, tryptophan (Trp) and Trp-derived secondary metabolites such as serotonin and 4-coumaroylserotonin accumulated in concert with methanol (MeOH) production. This senescence-induced MeOH induction was closely associated with levels of pectin methylesterase (PME)1 mRNA and PME enzyme activity. Exogenous challenge of detached rice leaves with 1% MeOH accelerated Trp and serotonin biosynthesis with induction of the corresponding genes. No other solvents including ethanol resulted in a Trp-inducing effect. This MeOH-induced Trp synthesis was positively regulated by abscisic acid but negatively regulated by cytokinin, suggesting hormonal involvement on the action of MeOH. Endogenous overproduction or suppression of MeOH either by PME1 overexpression or RNAi gene silencing revealed that PME1 overexpressing lines produced twofold higher Trp levels with elevated Trp biosynthetic gene expression, whereas RNAi lines showed twofold reduction in Trp level in healthy control rice leaves, suggesting that MeOH acts as an endogenous elicitor to enhance Trp biosynthesis. Among many transcription factors induced following MeOH treatment, the WRKY family showed significant induction patterns of which WRKY14 appeared to play a key regulatory role in MeOH-induced Trp and Trp-derived secondary metabolite biosynthesis.
Project description:We performed RNA-seq analysis of control, NHR-23-depleted, SPE-44-depleted and NHR-23+SPE-44 depleted adult male animals, to identify genes regulated by NHR-23 and SPE-44.