Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

GNPS Comparative metabolomics of diverse fungi crmA mutants

ABSTRACT: Comparative metabolomics of crmA mutants for Aspergillus fumigatus, Penicillium commune, P. camemberti, P. expansum, and Cochliobolus heterostrophus C5, co-culture of A. fumigatus and P. expansum, and deuterium labeling extractions

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Penicillium Expansum (ncbitaxon:27334) Aspergillus Fumigatus Af293 (ncbitaxon:330879) Penicillium Commune (ncbitaxon:36653) Cochliobolus Heterostrophus C5 (ncbitaxon:701091)

SUBMITTER: Frank Schroeder

PROVIDER: MSV000089206 | GNPS | Tue Apr 05 18:37:00 BST 2022

REPOSITORIES: GNPS

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Comparative metabolomics of diverse fungi crmA mutants

Project description:Comparative metabolomics of crmA mutants for Aspergillus fumigatus, Penicillium commune, P. camemberti, P. expansum, and Cochliobolus heterostrophus C5, co-culture of A. fumigatus and P. expansum, and deuterium labeling extractions

2022-04-05 | MSV000089206 | MassIVE

Proinflammatory cytokines and response to molds in mononuclear cells of patients with Meniere disease, vestibular migarine and Healthy controls

Project description:Analysis of PBMC gene expression level before and after stimulation with Aspergillus or Penicillium

2018-04-18 | GSE109558 | GEO

Comparative proteomics reveals energy and carbon metabolism changes in Scenedesmus quadricauda mutants induced by heavy-ion beam irradiation

Project description:the two contrasting mutants of Scenedesmus quadricauda were screened out after HIB mutagenesis. Integration of photosynthetic characteristics and comparative proteomic analysis revealed that the contrasting regulation of the TCA cycle proteins was showed in the two mutants.

2024-05-30 | PXD052737 |

The dynamic surface proteome of allergenic fungal conidia

Project description:Fungal spores and hyphal fragments are major contributors to the allergic response in the human lung. In this study, using a trypsin-shaving-proteomics approach, we identified the surface-exposed and secreted/shed proteins of Aspergillus fumigatus conidia and investigated the dynamics of the surface proteome under different conditions, including temperature variation and germination. We find that the surface proteome of resting A. fumigatus conidia is quite dynamic, as evidenced by drastically different surface proteomes under different growth conditions. We further investigated two observed A. fumigatus surface proteins, ScwA and CweA; ScwA is specific to the Aspergillus genus and only expressed on conidia grown at higher temperatures, whereas CweA is found throughout the Aspergillus and Penicillium genera. We extended our analysis of the surface proteome of A. fumigatus to other allergy-inducing pathogens; Alternaria alternata, Penicillium rubens, and Cladosporium herbarum, and performed comparative proteomics on resting and swollen conidia, as well as secreted proteins from germinating conidia. In this dataset, we detected 125 protein orthologs in the extracellular region of all four organisms, and 42 nonorthologous proteins produced by A. fumigatus, which may be useful in the development of future diagnostics. This study highlights the dynamic nature of the conidial surface and implicates growth conditions in the antigenicity of fungal conidia.

2020-01-24 | PXD017067 | Pride

Comparative genomic hybridization analysis in glycine max fast neutron mutants

Project description:Comparative genomic hybridization analysis in glycine max fast neutron mutants

| PRJNA486165 | ENA

Comparative smut fungi project

Project description:Sequencing and comparative genomics of smut fungi

| PRJEB19641 | ENA

Expression data from batch cultivations of Aspergillus niger wild type and adrA, facB and creA deletion mutants

Project description:Expression data from batch cultivations of Aspergillus niger wild type strain ATCC 1015 and adrA, facB and creA deletion mutants constructed on ATCC 1015 background strain with glucose or glycerol as carbon sources. Genome-wide transcriptome analysis was used to identify genes either affected directly or indirectly by each transcription factor investigated during growth on a repressing or a derepressing carbon source. For this purpose, batch cultivations under well-controlled conditions were performed with Aspergillus niger wild type strain ATCC 1015 and the three deletion mutants of the corresponding transcription factors AdrA, FacB and CreA. Samples for RNA extraction were collected and further processed for hybridization in custom-designed Affymetrix microarrays containing probes for three Aspergillus species, including A. niger.

2011-01-31 | GSE19952 | GEO

Time-course gene expression data from deletion strains of Penicillium chrysogenum

Project description:The recent discovery of a velvet complex containing several regulators of secondary metabolism in the model fungus Aspergillus nidulans raises the question whether similar type complexes direct fungal development in genera other than Aspergillus. Penicillium chrysogenum is the industrial producer of the antibiotic penicillin, whose biosynthetic regulation is barely understood. Here we provide a functional analysis of two major homologues of the velvet complex in P. chrysogenum, that we have named PcvelA and PclaeA. Data from array analysis using a ?PcvelA deletion strain indicate a significant role of PcvelA on the expression of biosynthesis and developmental genes, including PclaeA. Northern hybridization and HPLC quantifications of penicillin titres clearly show that both PcvelA and PclaeA play a major role in penicillin biosynthesis. Both regulators are further involved in different and distinct developmental processes. While PcvelA deletion leads to light independent conidial formation, dichotomous branching of hyphae and pellet formation in shaking cultures, a ?PclaeA strain shows a severe impairment in conidiophore formation in both the light and dark. Bimolecular fluorescence complementation assays finally provide evidence for a velvet-like complex in Penicillium chrysogenum, with structurally conserved components that have distinct developmental roles, illustrating the functional plasticity of these regulators within filamentous ascomycetes. Transcriptomes of PcvelA- and PclaeA- deletion mutants were compared with expression data from recipient strain deltaPcku70 and reference strain P2niaD18 as a control

2010-06-17 | E-GEOD-18585 | biostudies-arrayexpress

Comparative genomic, transcriptomic and secretomic profilings of Penicillium oxalicum HP7-1 and its cellulase and xylanase hyper-producing mutant EU2106, and identification of two novel regulators for cellulase and xylanase gene expression

Project description:In this study,comparative genomic, transcriptomic and secretomic profilings of Penicillium oxalicum HP7-1 and its cellulase and xylanase hyper-producing mutant EU2106 were employed to screen for novel regulators for cellulase and xylanase gene expression.

2016-08-31 | GSE80076 | GEO

MYC-mutants BioID

Project description:Proximity-based biotin labeling assay in HEK293 cells using FlagBirA-Myc (WT and mutants) fusion proteins.

2017-11-20 | MSV000081725 | MassIVE