Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

GNPS Burkholderia FERM BP-3421 dataset

ABSTRACT: This Dataset contains files investigating the metabolism of Burkholderia sp. FERM BP-3421. This whole set of data was collected and stored in Eustaquio Lab (the University of Illinois at Chicago - College of Pharmacy) and deposited on GNPS-Massive on 11/03/2022

INSTRUMENT(S): micrOTOF

ORGANISM(S): Burkholderia Sp. (ncbitaxon:36773)

SUBMITTER: Roger Linington Alessandra S Eustaquio Bruno Sacchetto Paulo

PROVIDER: MSV000090637 | GNPS | Thu Nov 03 12:44:00 GMT 2022

REPOSITORIES: GNPS

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Genome-Wide Mapping of Growth Regulated Genes in Burkholderia pseudomallei

Project description:Many microbial pathogens express specific virulence traits at distinct growth phases. To investigate the molecular pathways linking bacterial growth to pathogenicity, we characterized the genome-wide growth transcriptome of the tropical pathogen Burkholderia pseudomallei (Bp), the causative agent of melioidosis. Using fine-scale sampling, approximately 17% of all Bp genes were found to display regulated expression during growth, manifested primarily as discrete waves of gene expression tightly associated with distinct growth phases and transition points. A functional curation of these clusters provided evidence of a global ‘just-in-time’ production strategy to ensure the synthesis of molecular constituents only when needed. We observed regulation of multiple virulence factors at all growth phases, and by analyzing the early-phase transcriptome data, we identified and experimentally validated serC as a novel virulence factor in mice. Immunization of mice with serC-disrupted Bp also conferred protection against subsequent challenges with different wild-type Bp strains, demonstrating the potential utility of the serC mutant as an attenuated vaccine. We found a significant bias in early phase genes on Chromosome 1, supporting its proposed role as the ancestral Burkholderia chromosome, and utilized a chromosomally ordered co-expression metric to define ~100 putative operons throughout the Bp genome. These results extend our knowledge of virulence pathways in Bp, and suggest that molecular events at all growth phases, including early phase, are likely to play important roles in microbial pathogenicity." Keywords: Time Series Comparison Bp (K96243)

2006-10-01 | GSE5495 | GEO

Genomic Acquisition of Virulence Factors in Natural Isolates of Burkholderia

Project description:An important, but rarely performed, test of Koch’s molecular postulates involves evaluating the capacity of candidate virulence genes to confer pathogenicity in otherwise non-virulent species. Unbiased genomic surveys of avirulent natural isolates might reveal rare variants possessing specific virulence features, which might prove useful in testing their functional sufficiency. Using a custom pan-genome array, we analyzed a panel of avirulent Burkholderia thailandensis (Bt) isolates related to Burkholderia pseudomallei (Bp), the causative agent of the often fatal human and animal disease melioidosis. We report the discovery of variant Bt isolates exhibiting isolated acquisition of a capsular polysaccharide biosynthesis gene cluster (BpCPS), long regarded as an critical species-specific virulence factor essential for Bp mammalian virulence. BpCPS-expressing Bt strains exhibited certain pathogen-related phenotypes including resistance to human complement binding, but did not exhibit enhanced virulence when assessed in two different in vivo animal infection models. Phylogenetic analysis revealed that the BpCPS-expressing Bt strains likely reside within an evolutionary subgroup distinct from the majority of previously-described Bt strains. Our findings suggest that BpCPS acquisition alone is unlikely to fully explain the ability of Bp to colonize humans and animals, highlighting the importance of other collaborating factors in the pathogenesis of mammalian melioidosis.

2010-08-15 | GSE18766 | GEO

Comparative genomic hybridization of a Burkholderia cenocepacia collection

Project description:Individual normalized intensity of Burkholderia cenocepacia and Burkholderia seminalis hybridized in a B. cenocepacia array

2014-07-31 | GSE58353 | GEO

Burkholderia sp. FERM BP-3421

Project description:Burkholderia sp. FERM BP-3421 Genome sequencing

| PRJNA899914 | ENA

Burkholderia pseudomallei

Project description:Investigating the diversity of Burkholderia pseudomallei isolates

| PRJNA194126 | ENA

Burkholderia orbicola strain:2022

Project description:Burkholderia orbicola strain:2022 Genome sequencing