GNPS - Paullinia_cupana_massive_dataset_JosielAmazonas
Ontology highlight
ABSTRACT:
INSTRUMENT(S): micrOTOF-Q II
ORGANISM(S): Paullinia Cupana Var. Sorbilis (ncbitaxon:392746)
SUBMITTER: 
Josiel do Nascimento Amazonas
PROVIDER: MSV000091217 | GNPS | Tue Feb 07 13:02:00 GMT 2023
REPOSITORIES: GNPS
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Project description:The guarana extracts were submitted to liquid chromatography analysis coupled with mass spectrometry at the Analytical Center of the Thematic Laboratory of Chemistry of Natural Products-CALTQPN at INPA-Brazil.
The extract samples obtained were prepared by weighing them in Eppendorf tubes and solubilizing them in a mixture of MeOH (0.1% formic acid) and 0.1% formic acid solution, in a 1:1 ratio, having the final concentration of 1 mg/mL. Finally, the tubes were centrifuged and transferred to vials for further analysis.
The equipment used was a Liquid Chromatograph (Shimadzu, Japan) coupled to a high-resolution Time-of-flight mass spectrometer (MicroTOF QII model, Bruker Daltonics, Bremen, Germany), with an ESI-type ionization source in negative mode. For analysis, a Luna 5u PFP2 100A column (15 cm x 2.00 mm) was used and the mobile phases with the solvents: A: 0.1% formic acid solution.
MS/MS parameters: capillary: 3500 volts; Scan: 120-1200m/z; Set nebulizer: 2Bar psi; Set dry Heater: 180 Celsius; Set dry gas: 9.0 L/ min.; Rolling Averages: 1cts.
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Project description:The guarana extracts were submitted to liquid chromatography analysis coupled with mass spectrometry at the Analytical Center of the Thematic Laboratory of Chemistry of Natural Products-CALTQPN at INPA-Brazil. The extract samples obtained were prepared by weighing them in Eppendorf tubes and solubilizing them in a mixture of MeOH (0.1% formic acid) and 0.1% formic acid solution, in a 1:1 ratio, having the final concentration of 1 mg/mL. Finally, the tubes were centrifuged and transferred to vials for further analysis. The equipment used was a Liquid Chromatograph (Shimadzu, Japan) coupled to a high-resolution Time-of-flight mass spectrometer (MicroTOF QII model, Bruker Daltonics, Bremen, Germany), with an ESI-type ionization source in negative mode. For analysis, a Luna 5u PFP2 100A column (15 cm x 2.00 mm) was used and the mobile phases with the solvents: A: 0.1% formic acid solution. MS/MS parameters: capillary: 3500 volts; Scan: 120-1200m/z; Set nebulizer: 2Bar psi; Set dry Heater: 180 Celsius; Set dry gas: 9.0 L/ min.; Rolling Averages: 1cts.
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Project description:The guarana extracts were submitted to liquid chromatography analysis coupled with mass spectrometry at the Analytical Center of the Thematic Laboratory of Chemistry of Natural Products-CALTQPN at INPA-Brazil.
The extract samples obtained were prepared by weighing them in Eppendorf tubes and solubilizing them in a mixture of MeOH (0.1% formic acid) and 0.1% formic acid solution, in a 1:1 ratio, having the final concentration of 1 mg/mL. Finally, the tubes were centrifuged and transferred to vials for further analysis.
The equipment used was a Liquid Chromatograph (Shimadzu, Japan) coupled to a high-resolution Time-of-flight mass spectrometer (MicroTOF QII model, Bruker Daltonics, Bremen, Germany), with an ESI-type ionization source in negative mode. For analysis, a Luna 5u PFP2 100A column (15 cm x 2.00 mm) was used and the mobile phases with the solvents: A: 0.1% formic acid solution.
MS/MS parameters: capillary: 3500 volts; Scan: 120-1200m/z; Set nebulizer: 2Bar psi; Set dry Heater: 180 Celsius; Set dry gas: 9.0 L/ min.; Rolling Averages: 1cts.
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62-70m _8%
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Luna5uPFP2;100A_15X2
Flx=0,1875 mL/minSSplit; P:1026 psi;F:30C;
C=1mg/mL(MeOH/H2O_1:1_0.1%ACF);Inj=10uL;
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