Project description:Untargeted metabolomics of Botrytis-Bacillus interaction at 6,24 and 48h. Ethyl-acetate extraction of supernatant fraction and methanol extraction for cell fraction.
Project description:Samples from monoculture of bacteria or fungi grown for 24h. Samples were extracted with methanol 80% or ethyl-acetate (supernatant fractions)
Project description:Transcriptome sequencing of mice treated with Chebulae Fructus hydrosoluble extract ethyl acetate fraction and water were performed, and the gene expression profiles were compared.
Project description:Three independent cultures of Methylorubrum extorquens PA1 delta cel were grown on ammonium mineral salts with either methanol or succinate provided as the sole carbon and energy source. The supernatant was subsequently extracted with acidified ethyl acetate and analyzed by LC-MS.
Project description:PKS14-overexpressing strain was inoculated into PDB for 7 days. The fungal cells were extracted with methanol, and the culture broth was extracted with ethyl acetate.
Project description:The PKS14-knockout strain was inoculated into PDB for 7 days. The fungal cells were extracted with methanol, and the culture broth was extracted with ethyl acetate.
Project description:We performed an untargeted metabolomic analysis on agave tequilana leaves treated with two phyllosphere synthetic microbial communities (PSMC) using LC-HR-MS/MS. Data-Dependent Acquisition was employed under positive ionization mode. Three cohorts were included; a) non-treated plants; b) treated plants with PFCS (a type of PSMC), and; c) treated plants with PFCT (a type of PSMC).
Metabolites were extracted with a solvent mixture of acetonitrile:methanol:ethyl acetate (1:1:1).