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GNPS_Bile_acid_modification_fecal_samples_and_synthetic standards of polyamine amidates

ABSTRACT: Mouse and carnivore animal fecal samples obtained from Chris Turck (original dataset: MSV000080574) and Itzik Mizrahi (original dataset: MSV000086131). We recollected untargeted LC-MS/MS on these samples on a Vanquish UHPLC system coupled to a Q-Exactive Hybrid Quadrupole-Orbitrap (Thermo Fisher Scientific, Bremen, Germany). Two different chromatographic separation were performed on a Kinetex 2.6 um 100 A pore size C18 reversed phase UHPLC column 100 x 2.1 mm (Phenomenex, Torrance, CA). Extracted fecal samples were also spiked with synthetic standards of polyamine bile acid amidates (in different concentrations) to perform retention time matching.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus <mouse, Genus> (ncbitaxon:10088) Mammalia (ncbitaxon:40674)

SUBMITTER: Pieter C Dorrestein

PROVIDER: MSV000091663 | GNPS | Sat Apr 08 00:35:00 BST 2023

REPOSITORIES: GNPS

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