Metabolomics

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GNPS - Root exudate metabolomics data for Brachypodium distachyon in fabricated ecosystems (EcoFAB 2.0)

ABSTRACT: Root exudate LC-MS data for Brachypodium distachyon Bd21-3 grown for 5 weeks on different iN+ media (groups: +NH4, +NO3, +NH4NO3) or 3 weeks in iN+ medium followed by two weeks on iN-free medium (groups: -NH4, -NO3, -NH4NO3). Each treatment had 5 biological replicates. The iN+ media contained inorganic N (iN) at 5 mM. Plants were grown inside EcoFAB 2.0.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Brachypodium Distachyon (ncbitaxon:15368)

SUBMITTER: Trent Northen  

PROVIDER: MSV000092053 | GNPS | Tue May 30 12:17:00 BST 2023

REPOSITORIES: GNPS

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Publications

Reproducible growth of <i>Brachypodium</i> in EcoFAB 2.0 reveals that nitrogen form and starvation modulate root exudation.

Novak Vlastimil V   Andeer Peter F PF   Bowen Benjamin P BP   Ding Yezhang Y   Zhalnina Kateryna K   Hofmockel Kirsten S KS   Tomaka Connor C   Harwood Thomas V TV   van Winden Michelle C M MCM   Golini Amber N AN   Kosina Suzanne M SM   Northen Trent R TR  

Science advances 20240103 1

Understanding plant-microbe interactions requires examination of root exudation under nutrient stress using standardized and reproducible experimental systems. We grew <i>Brachypodium distachyon</i> hydroponically in fabricated ecosystem devices (EcoFAB 2.0) under three inorganic nitrogen forms (nitrate, ammonium, and ammonium nitrate), followed by nitrogen starvation. Analyses of exudates with liquid chromatography-tandem mass spectrometry, biomass, medium pH, and nitrogen uptake showed EcoFAB  ...[more]

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