Dataset Information

GNPS - Carnitine analyses of hylid tree frogs (Amphibia: Anura)

ABSTRACT: Samples were obtained from the dorsal skin of frogs after a mild electrical stimuli. They were freeze-dried and resuspended in water: acetonitrile (7:3) with 0.1% (v/v) trifluoroacetic acid (TFA). The aim of the analyses was to identified secondary metabolites in particular from the Boana pulchella group. After isolation and characterization of two novel macrocyclic acylcarnitines (MACH) by a combination of LC-MS/MS, RMN and GC/MS we also putatively identified other substances from the same series.

INSTRUMENT(S): micrOTOF-Q II

ORGANISM(S): Bokermannohyla Astartea (ncbitaxon:279988) Boana Prasina (ncbitaxon:2485001) Boana Caingua (ncbitaxon:2599766) Dendropsophus Norandinus Boana Semilineata (ncbitaxon:2339192) Xenohyla Truncata (ncbitaxon:318415) Dendropsophus Molitor Hyloscirtus Larinopygion (ncbitaxon:1216023) Dendropsophus Minutus (ncbitaxon:150711) Trachycephalus Typhonius (ncbitaxon:1033724) Boana Lundii (ncbitaxon:2517092) Boana Pulchella (ncbitaxon:280005) Boana Stellae (ncbitaxon:2494687) Scinax Fuscovarius (ncbitaxon:318393) Dendropsophus Bogerti Bokermannohyla Luctuosa Nyctimantis Brunoi Aplastodiscus Perviridis (ncbitaxon:318259) Dendropsophus Nanus (ncbitaxon:280003) Hyloscirtus Bogotensis Boana Faber (ncbitaxon:279995) Boana Bischoffi (ncbitaxon:279990) Aplastodiscus Leucopygius (ncbitaxon:317334) Boana Polytaenia Boana Albomarginata (ncbitaxon:212358) Boana Albopunctata (ncbitaxon:279985) Phyllomedusa Burmeisteri (ncbitaxon:39413) Bokermannohyla Circumdata (ncbitaxon:279992) Pithecopus Hypochondrialis Boana Punctata (ncbitaxon:2499473)

SUBMITTER: Andres Eduardo Brunetti

PROVIDER: MSV000092499 | GNPS | Sat Jul 22 13:59:00 BST 2023

REPOSITORIES: GNPS

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:Along with the prevalence of edible frog farming in China, the outbreak of a deadly infectious frog diseased, called frog meningitis (or cataracts and torticollis), has increased in frequency and geographical range dramatically. More than 10 bacterial species, belonging to 8 genera, has been reported as its potential pathogens. Diseased frogs typically manifest as torticollis, cataracts, edema and finally death, resulting in huge economic loss. Currently, the pathogenesis of this disease has not been investigated systematically. Here, we summarized the pathological stages of infected black-spotted frogs (Pelophylax nigromaculata) in Sichuan province according to their symptoms, typically progressing of pathological stage with only torticollis to stage with both torticollis and cataracts. On the basis, we analyzed the pathogenesis by a combination of comparative environmental analysis, microbiomics and transcriptomics. Results showed that more severely infected frog ponds tended to have lower water alkalinity. Elizabethkingia miricola was the only bacteria, whose abundance was positively correlated with the disease degree, and it has absolute dominance in the eyeball and brain of some torticollis-cataracts frogs. E. miricola and several other bacterial species, which belonged to pathogenic genera of meningitis, might be constitutively existed in the resident microbiome in frogs or their environment. Activations of infectious processes and immune responses related pathways were the major difference between health and diseased frogs at transcriptional level. Despite transcriptional activation of immunoglobulins was observed in both torticollis-only and torticollis-cataracts frogs, transcriptional activation of innate immune system (including MHC, toll-like receptor, and cathelicidins) in brain, inflammation system (including interleukins and receptors) in brain, and acute phase proteins (including transferrins and fibrinogens) in both liver and brain was only observed in torticollis-cataracts frogs. Activation of inflammation and the resulting higher vascular permeability in torticollis-cataracts frogs could explain the severe brain infection, cooccurrence of torticollis and cataracts, and systemic edema in torticollis-cataracts frogs. In addition, meningitis could also result in reduction in energy production in liver, and this was more severe in torticollis-cataracts frogs. In conclusion, our results suggested environment might have a role in susceptibility of frog meningitis. E. miricola was the most likely pathogen of meningitis of black-spotted frogs in Sichuan. Refer to the pathogenesis of human meningitis, excessive inflammation likely played a critical role in the progress of frog meningitis, and its resulted sepsis and organ failure might be the direct cause of infected frogs.

Project description:These data provide a basis for exploration of gene expression differences between physiologically extreme accessions of Arabidopsis thaliana. Recent studies have documented remarkable genetic variation among Arabidopsis thaliana accessions collected from diverse habitats and across its geographical range. Of particular interest are accessions with putatively locally adapted phenotypes â i.e., accessions with attributes that are likely adaptive under the climatic or habitat conditions of their sites of origin. These genotypes are especially valuable as they may provide insight into the genetic basis of adaptive evolution as well as allow the discovery of genes of ecological importance. Therefore we studied the physiology, genome content and gene expression of two physiologically extreme accessions (Tsu-1 from a wet environment in Tsushima, Japan and Kas-1 from a dry environment in Kashmir, India). The gene expression studies were conducted under two levels of soil moisture and accompanied by physiological measurements to characterize early responses to soil moisture deficit. Genomic hybridization experiments identified 42,503 single feature polymorphisms (SFP) between accessions, providing an initial screen for putative SNPs, indels, or changes in gene content. Microarray transcript profiling of leaf RNA identified a large number (5,996) of genes exhibiting robust constitutive differences in expression including many genes involved in general growth and development as well as abiotic stress pathways. Interestingly, mild soil drying resulted in only subtle physiological responses but resulted in gene expression changes in hundreds of transcripts, including 352 genes exhibiting differential responses between accessions. Our results highlight the value of genomic studies of natural accessions as well as identify a number of candidate genes for the observed constitutive and induced physiological differences between Tsu-1 and Kas-1. The basic experimental design involves two accessions (Tsu-1 and Kas-1) crossed with two environmental levels (well-watered soil and mild soil drying) and 3 biological replicates per accession/treatment combination (2 x 2 x 6 =24 arrays total)

Dataset Information

GNPS - Carnitine analyses of hylid tree frogs (Amphibia: Anura)

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets