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GNPS - azodyrecin biosynthesis study (CeMiSt)

ABSTRACT: Investigation of azodyrecin biosynthesis through heterologous expression and knockout studies in wild type producer (Streptomyces mirabilis P8-A2). FBMN conducted and preprocessing with MS-DIAL.

INSTRUMENT(S): 6545 Q-TOF LC/MS

ORGANISM(S): Streptomyces Albidoflavus (ncbitaxon:1886) Streptomyces Coelicolor (ncbitaxon:1902) Streptomyces Mirabilis (ncbitaxon:68239)

SUBMITTER: Ling Ding Karl Tilmann Weber Matiss Maleckis

PROVIDER: MSV000092718 | GNPS | Tue Aug 22 02:25:00 BST 2023

REPOSITORIES: GNPS

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Biosynthesis of the Azoxy Compound Azodyrecin from Streptomyces mirabilis P8-A2.

Maleckis Matiss M Wibowo Mario M Gren Tetiana T Jarmusch Scott A SA Sterndorff Eva B EB Booth Thomas T Henriksen Nathalie N S E NNSE Whitford Christopher M CM Jiang Xinglin X Jørgensen Tue S TS Ding Ling L Weber Tilmann T

ACS chemical biology 20240210 3

Azoxy compounds are a distinctive group of bioactive secondary metabolites characterized by a unique RN═N+(O-)R moiety. The azoxy moiety is present in various classes of metabolites that exhibit various biological activities. The enzymatic mechanisms underlying azoxy bond formation remain enigmatic. Azodyrecins are cytotoxic azoxy metabolites produced by Streptomyces mirabilis P8-A2. Here, we cloned and confirmed the putative azd biosynthetic gene cluster thro ...[more]

PMID: 38340355

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