Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

GNPS - Standards of bile acids (2) on Q Exactive

ABSTRACT: MS/MS fragmentation data of bile acid standards acquired on the QE - with chromatographic separation on a Phenomenex polar C18 column and MS2 fragmentation at NCE 45.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Na

SUBMITTER: Pieter C Dorrestein

PROVIDER: MSV000092984 | GNPS |

REPOSITORIES: GNPS

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Standards of bile acids on Q Exactive

Project description:MS/MS fragmentation data on bile acid standards were acquired on the QE - with a gradient developed to separate between isomeric pairs on a Polar C18 column and a fragmentation energy of NCE 45.

2023-05-22 | MSV000092003 | MassIVE

GNPS - Standards of bile acids on Q Exactive

2023-05-22 | MSV000092003 | GNPS

NADLF and bile acids

Project description:NADLF and bile acids Raw sequence reads

| PRJNA817288 | ENA

Effect of Bile Acids on C. difficile Growth

Project description:Clostridium difficile epidemic strain R20291 was grown in presence of the two main bile acids, Deoxycholate and Cholate.

2019-08-28 | GSE86152 | GEO

Bile acids and gut motility

Project description:Microbiome-encoded bile acid metabolism modulates colonic transit times

| PRJEB38547 | ENA

Bile Salt Hydrolase Amino Transfer to Bile Acids

Project description:Microbiome Data from mice gavaged with different bile acids

| PRJEB67375 | ENA

A systems-level analysis of bile acids effects on rat colon epithelial cells

Project description:Bile acid diarrhoea is a chronic condition caused by increased delivery of bile acids to the colon. The underlying mechanisms remain to be elucidated. To investigate genes involved in bile acid diarrhoea, systems-level analyses were employed on a rat bile acid diarrhoea model. Twelve male Wistar Munich rats, housed in metabolic cages, were fed either control or bile acid-mixed (1% w/w) diets for ten days. Food intake, water intake, urine volume, bodyweight and faecal output were monitored daily. After euthanasia, colonic epithelial cells were isolated using calcium-chelation and processed for systems-level analyses, i.e. RNA-sequencing transcriptomics and mass spectrometry proteomics. Bile acid-fed rats suffered diarrhoea, indicated by increased drinking, faeces weight and faecal water content compared with control rats. Urine output was unchanged. With bile acid-feeding, RNA-sequencing revealed 204 increased and 401 decreased mRNAs; mass spectrometry 183 increased and 111 decreased proteins. Among the altered genes were genes associated with electrolyte and water transport (including Slc12a7, Clca4 and Aqp3) and genes associated with bile acid transport (Slc2b1, Abcg2, Slc51a, Slc51b and Fabps). Correlation analysis showed a significant positive correlation (Pearson’s r=0.28) between changes in mRNA-expression and changes in protein-expression. However, caution must be exercised in making a direct correlation between experimentally determined transcriptomes and proteomes. Genes associated with bile acid transport responded to bile acid-feeding, suggesting that colonic bile acid transport occur by regulated protein facilitated mechanisms rather than passive diffusion. In addition, the study provides annotated rat colonic epithelial cell transcriptome and proteome with response to bile acid-feeding.

2024-05-10 | GSE174249 | GEO

Response to bile of Lactobacillus casei BL23

Project description:The response to bile of Lactobacillus casei BL23 was investigated. Lactobacillus casei BL23 was grown in MRS medium at 37C without shaking until O.D. 595 nm. reached 0.5. The cultures were then two fold diluted with prewarmed MRS medium (control) or MRS supplemented with 0.2% bovine bile (treatment) and incubation continued for 45 min. Cells were then harvested and total RNA purified.

2011-10-19 | E-GEOD-33047 | biostudies-arrayexpress

Differential view on the bile acids stress response of Clostridioides difficile

Project description:In this study, we aimed at the characterization of C. difficile’s stress response to the main four human bile acids. Although, a phenotypically description of growth differences upon challenge with different bile acids has been described (Lewis 2016, Thanissery 2017), there is no information on the adaptation of gene expression available. We employed a comprehensive proteomics approach to record stress signatures of the unconjugated bile acids CA, CDCA, DCA and LCA in shock experiments as well as during long-term-stress conditions and could depict a general stress response concerning all four bile acids, but also specific responses to only a single or a few of the different bile acids. Our results are a starting point for the understanding of how the individual bile acids cocktail of a patient can decide on the outcome of a C. difficile infection.

2019-02-12 | PXD010514 | Pride

CMMC_bile_acid_amino_acid_conjugates

Project description:MS/MS fragmentation data of 23 different bile acid steroid core conjugated with amino acids acquired on Q Exactive (positive ionization mode)-with chromatographic separation on a Phenomenex polar C18 column. The data for each reaction mixture is acquired on stepped collision energy and at only NCE 45.

2024-04-17 | MSV000094561 | MassIVE

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data